Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
2.2 A resolution structure reveals a bacterial methyltransferase domain joined to a eukaryotic kinase domain. The kinase domain is again fused to an extended C-terminal coiled-coil domain reminiscent of eukaryotic Myotonic Dystrophy Protein Kinase family kinases. Structures of co-complexes with two known eukaryotic protein kinase inhibitors which are potent inhibitors in vitro, but do not show any in vivo activity | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D350A | 88.8% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 95.2% with substrate D-mannose | Escherichia coli |
D351A | 0.9% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.1% with substrate D-mannose | Escherichia coli |
D351E | 4.5% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.5% with substrate D-mannose | Escherichia coli |
DELTA398-418 | 6.7% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 4.7% with substrate D-mannose | Escherichia coli |
E274A | 45.4% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 66.6% with substrate D-mannose | Escherichia coli |
H132A | complete loss of activity | Escherichia coli |
H133A | complete loss of activity | Escherichia coli |
N34D/Q35E/H197E | complete loss of activity | Escherichia coli |
R203A | complete loss of activity | Escherichia coli |
R270A | 72.8% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 85.7% with substrate D-mannose | Escherichia coli |
W355F | 72.4% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 1.9% with substrate D-mannose | Escherichia coli |
W355H | 48.7% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 50.7% with substrate D-mannose | Escherichia coli |
Y16F | complete loss of activity | Escherichia coli |
Y230F | 0.1% of wild-type activity with substrate 2-alpha-D-mannosyl-D-mannose, 2.5% with substrate D-mannose | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | J7I4B7 | - |
- |
Escherichia coli O9a | J7I4B7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-alpha-D-mannosyl-D-mannose | - |
Escherichia coli | ADP + 3-O-phospho-2-alpha-D-mannosyl-D-mannose | - |
? | |
ATP + 2-alpha-D-mannosyl-D-mannose | - |
Escherichia coli O9a | ADP + 3-O-phospho-2-alpha-D-mannosyl-D-mannose | - |
? | |
ATP + D-mannose | - |
Escherichia coli | ADP + 3-O-phospho-D-mannose | - |
? | |
ATP + D-mannose | - |
Escherichia coli O9a | ADP + 3-O-phospho-D-mannose | - |
? |