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Literature summary for 2.7.1.173 extracted from
- Crystal structure of human nicotinamide riboside kinase (2007), Structure, 15, 1005-1013.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of His-tagged NRK1 in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| NRK1 in complex with the reaction product nicotinamide mononucleotide, and NRK1 in a ternary complex with ADP and the anticancer drug tiazofurin, sitting-drop vapor-diffusion method, 22°C, mixing of protein solution containing 20 mg/ml protein, 2 mM ligand, 20 mM Tris, pH 7.5-7.8, 200 mM NaCl, 5 mM DTT, with reservoir solution containing 28% w/v PEG 3350, 200 mM NH4Cl, 5 mM DTT, and 5 mM Na2HPO4, X-ray diffraction structure determination and analysis at 1.5 A and 2.7 A resolution, respectively, modeling | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-D-ribosylnicotinamide | Homo sapiens | - |
ADP + nicotinamide beta-D-ribonucleotide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9NWW6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged NRK1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + beta-D-ribosylnicotinate = ADP + nicotinate beta-D-ribonucleotide | catalytic mechanism, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-D-ribosylnicotinamide | - |
Homo sapiens | ADP + nicotinamide beta-D-ribonucleotide | - |
? | |
| ATP + nicotinamide mononucleotide | binding mode of the substrate, overview | Homo sapiens | ADP + nicotinamide mononucleotide phosphate | - |
? | |
| ATP + tiazofurin | binding mode of the substrate, overview | Homo sapiens | ADP + ? | - |
? | |
| additional information | ADP binding mode, overview | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the structure of NRK1 contains a central, five-stranded parallel beta sheet, with five alpha helices on both faces, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nicotinamide riboside kinase | - |
Homo sapiens |
| Nrk | - |
Homo sapiens |
| NRK1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | nicotinamide riboside kinase has an important role in the biosynthesis of NAD+ as well as the activation of tiazofurin and other nicotinamide riboside analogues for anticancer therapy | Homo sapiens |
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Release 2023.1 (January 2023)
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