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Literature summary for 2.7.1.172 extracted from

  • Payne, L.S.; Brown, P.M.; Middleditch, M.; Baker, E.; Cooper, G.J.; Loomes, K.M.
    Mapping of the ATP-binding domain of human fructosamine 3-kinase-related protein by affinity labelling with 5'-[p-(fluorosulfonyl)benzoyl]adenosine (2008), Biochem. J., 416, 281-288.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5'-p-[(fluorosulfonyl)benzoyl]adenosine incubation of 0.7 mg/ml Fn3KRP with 0.5 mM inhibitor results in significant activity loss compared with controls. The inhibitor inactivates Fn3KRP by modification of residues at the nucleotide-binding site Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
[lysozyme]-N6-D-psicosyl-L-lysine pH 7.8, 30°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HA64
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
lung cancer cell line
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [lysozyme]-N6-D-psicosyl-L-lysine in contrast with free psicose-lysine, not all psicosamine residues in the glycated lysozyme substrate would be expected to be accessible to the Fn3KRP active site. In addition, local charge effects may influence the relative affinity for psicosamine residues at different sites in the lysozyme substrate Homo sapiens ADP + [lysozyme]-N6-(O3-phosphono-D-psicosyl)-L-lysine
-
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Synonyms

Synonyms Comment Organism
FN3K-related protein
-
Homo sapiens
Fn3KRP
-
Homo sapiens
fructosamine 3-kinase-related protein
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Homo sapiens