KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
[protein]-N6-D-fructosyl-L-lysine | pH 8.0, 37°C | Homo sapiens | |
0.5 | - |
N5-D-fructosyl-L-ornithine | pH 8.0, 37°C | Homo sapiens | |
0.75 | - |
N6-D-fructosyl-L-lysine | pH 8.0, 37°C | Homo sapiens | |
50 | - |
D-fructose | pH 8.0, 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, calculated from sequence | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [protein]-N6-D-fructosyl-L-lysine | Homo sapiens | nonenzymatic glycation is an important factor in the pathogenesis of diabetic complications. Key early intermediates in this process are fructosamines, such as protein-bound fructoselysines. The fructosamine most frequently encountered in nature is fructoselysine. Fructosamine-3-kinase is part of an ATP-dependent system for removing carbohydrates from nonenzymatically glycated proteins and protecting cells from the deleterious effects of nonenzymatic glycation | ADP + [protein]-N6-(O3-phosphono-D-fructosyl)-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + [protein]-N6-D-fructosyl-L-lysine = ADP + [protein]-N6-(3-O-phospho-D-fructosyl)-L-lysine | phosphorylation destablilizes the fructoselysine adduct and leads to its spontaneous decomposition | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
kidney | the enzyme is present in all tissues, with the highest level of expression in the kidney | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.08 | - |
pH 8.0, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | Vmax is 35% of the value for N6-D-fructosyl-L-lysine | Homo sapiens | ADP + O3-phosphono-D-fructose | - |
? | |
ATP + N5-D-fructosyl-L-ornithine | - |
Homo sapiens | ADP + N5-(O3-phosphono-D-fructosyl)-L-ornithine | - |
? | |
ATP + N6-D-fructosyl-L-lysine | - |
Homo sapiens | ADP + N6-(O3-phosphono-D-fructosyl)-L-lysine | - |
? | |
ATP + [protein]-N6-D-fructosyl-L-lysine | nonenzymatic glycation is an important factor in the pathogenesis of diabetic complications. Key early intermediates in this process are fructosamines, such as protein-bound fructoselysines. The fructosamine most frequently encountered in nature is fructoselysine. Fructosamine-3-kinase is part of an ATP-dependent system for removing carbohydrates from nonenzymatically glycated proteins and protecting cells from the deleterious effects of nonenzymatic glycation | Homo sapiens | ADP + [protein]-N6-(O3-phosphono-D-fructosyl)-L-lysine | - |
? | |
ATP + [protein]-N6-D-fructosyl-L-lysine | [histone]-N6-D-fructosyl-L-lysine, [hemoglobin]-N6-D-fructosyl-L-lysine. Similar experiments with other glycated proteins, including bovine serum albumin, and lysozyme indicate that fructoselysine residues on glycated proteins are readily phosphorylated by fructosamine 3-kinase, apparently irrespective of the protein. Phosphorylation destablilizes the fructoselysine adduct and leads to its spontaneous decomposition | Homo sapiens | ADP + [protein]-N6-(O3-phosphono-D-fructosyl)-L-lysine | - |
? | |
additional information | the kinase is specific for 1-deoxy-1-amino fructose adducts and does not catalyze phosphorylation of other monosaccharides and polyols, such as glucose, galactose, mannose, glucosamine, galactosamine, or myo-inositol | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, calculated from sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
FN3K | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |