Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activation, better than Mg2+ | Lactiplantibacillus pentosus | |
Mg2+ | activation, equally effective as Mn2+ or Zn2+ | Lactiplantibacillus pentosus | |
Mn2+ | activation is as effective as with Mg2+, equally effective as Zn2+ or Mg2+ | Lactiplantibacillus pentosus | |
Zn2+ | activation, equally effective as Mg2+ or Mn2+ | Lactiplantibacillus pentosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus pentosus | - |
- |
- |
Lactiplantibacillus pentosus 124-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Lactiplantibacillus pentosus |
Storage Stability | Organism |
---|---|
0°C, several months | Lactiplantibacillus pentosus |
4°C, 1 mg protein/ml in 0.1 M potassium phosphate buffer, pH 7, 2 mM MgCl2, 0.1 mM EDTA, 0.1 mM PMSF, 10 mM 2-mercaptoethanol, 90% loss of activity within 18 h | Lactiplantibacillus pentosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-xylulose | specific for D-isomer | Lactiplantibacillus pentosus | ADP + D-xylulose 5-phosphate | - |
r | |
ATP + D-xylulose | specific for D-isomer | Lactiplantibacillus pentosus 124-2 | ADP + D-xylulose 5-phosphate | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
5 min, 66%, 60%, 50%, 30% or 26% loss of activity at pH 8, pH 7, pH 6.2, pH 5.7 and pH 5.4, respectively | Lactiplantibacillus pentosus |
60 | - |
5 min, inactivation at pH 5.4 | Lactiplantibacillus pentosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.9 | - |
Lactiplantibacillus pentosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
5 min, 26% loss of activity at 53°C, inactivation at 60°C | Lactiplantibacillus pentosus |
5.7 | - |
5 min, 30% loss of activity at 53°C | Lactiplantibacillus pentosus |
6.2 | - |
5 min, 50% loss of activity at 53°C | Lactiplantibacillus pentosus |
7 | - |
5 min, 60% loss of activity at 53°C | Lactiplantibacillus pentosus |
8 | - |
5 min, 66% loss of activity at 53°C | Lactiplantibacillus pentosus |