Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermoproteus tenax |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-glycerate | substrate inhibition at high glycerate concentrations | Thermoproteus tenax | |
ADP | competitive inhibition | Thermoproteus tenax | |
EDTA | 40 mM | Thermoproteus tenax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
(R)-glycerate | - |
Thermoproteus tenax | |
0.03 | - |
ATP | - |
Thermoproteus tenax |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 15% of the activity with Mg2+ | Thermoproteus tenax | |
Co2+ | 56% of the activity with Mg2+ | Thermoproteus tenax | |
Mg2+ | highest activity is observed in presence of 20 mM Mg2+ | Thermoproteus tenax | |
Mn2+ | 59% of the activity with Mg2+ | Thermoproteus tenax | |
additional information | no activity is detected in the presence of Cu2+ and Fe2+ at 0.2, 2, 5 and 20 mM, respectively | Thermoproteus tenax | |
Ni2+ | 30% of the activity with Mg2+ | Thermoproteus tenax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
1 * 44000, SDS-PAGE | Thermoproteus tenax |
48300 | - |
gel filtration | Thermoproteus tenax |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-glycerate | Thermoproteus tenax | enzyme of the branched Entner-Doudoroff pathway | ADP + 2-phospho-(R)-glycerate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoproteus tenax | Q703V9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoproteus tenax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-glycerate | - |
Thermoproteus tenax | ADP + 2-phospho-(R)-glycerate | - |
? | |
ATP + (R)-glycerate | enzyme of the branched Entner-Doudoroff pathway | Thermoproteus tenax | ADP + 2-phospho-(R)-glycerate | - |
? | |
CTP + (R)-glycerate | 16% of the activity with ATP | Thermoproteus tenax | CDP + 2-phospho-(R)-glycerate | - |
? | |
GTP + (R)-glycerate | 20% of the activity with ATP | Thermoproteus tenax | GDP + 2-phospho-(R)-glycerate | - |
? | |
additional information | no activity is observed with ADP, diphosphate and polyphosphates | Thermoproteus tenax | ? | - |
? | |
TTP + (R)-glycerate | 16% of the activity with ATP | Thermoproteus tenax | TDP + 2-phospho-(R)-glycerate | - |
? | |
UTP + (R)-glycerate | 10% of the activity with ATP | Thermoproteus tenax | UDP + 2-phospho-(R)-glycerate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, SDS-PAGE | Thermoproteus tenax |
Synonyms | Comment | Organism |
---|---|---|
garK | - |
Thermoproteus tenax |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermoproteus tenax |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | 50°C: 15% of maximal activity, 70°C: 55% of maximal activity, 90°C: maximal activity | Thermoproteus tenax |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
ATP | - |
Thermoproteus tenax | |
3.6 | - |
D-glycerate | - |
Thermoproteus tenax |