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Literature summary for 2.7.1.165 extracted from

  • Reher, M.; Bott, M.; Schoenheit, P.
    Characterization of glycerate kinase (2-phosphoglycerate forming), a key enzyme of the nonphosphorylative Entner-Doudoroff pathway, from the thermoacidophilic euryarchaeon Picrophilus torridus (2006), FEMS Microbiol. Lett., 259, 113-119.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
(R)-glycerate
-
Picrophilus torridus
0.5
-
ATP
-
Picrophilus torridus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 0.05 mM, most effective divalent cation Picrophilus torridus
additional information Mg2+ can be replaced to some extent by Ni2+ (25%), Mn2+ (11%), and Co2+ (11%) Picrophilus torridus
additional information no activating effect of K+ Picrophilus torridus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47500
-
2 * 47500, gel filtration Picrophilus torridus
50000
-
SDS-PAGE, denatured enzyme Picrophilus torridus
93000
-
calculated from amino acid sequence Picrophilus torridus
95000
-
gel filtration, native enzyme Picrophilus torridus

Organism

Organism UniProt Comment Textmining
Picrophilus torridus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Resource Phenyl column chromatography, Superdex 200 HiLoad 16/60 column gel filtration, and UNO-Q1 column chromatography Picrophilus torridus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.07
-
cell extract, at 50°C Picrophilus torridus
358
-
after 5114fold purification, at 50°C Picrophilus torridus

Storage Stability

Storage Stability Organism
60°C, 100 mM Tris-HCl at pH 8.0, 2 h, no loss of activity Picrophilus torridus
70°C, 100 mM Tris-HCl at pH 8.0, 5 min, 50% loss of activity Picrophilus torridus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-glycerate
-
Picrophilus torridus ADP + 3-phospho-(R)-glycerate
-
?
additional information the enzyme shows highest activity with D-glycerate (100%), and lower activity (34%) with L-glycerate Picrophilus torridus ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 47500, gel filtration Picrophilus torridus

Synonyms

Synonyms Comment Organism
GCK
-
Picrophilus torridus
glycerate kinase (2-phosphoglycerate forming)
-
Picrophilus torridus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Picrophilus torridus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
Picrophilus torridus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.6 8.6 50% of activity at pH 5.6 and at pH 8.6 Picrophilus torridus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Picrophilus torridus