Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no increased activity in the presence of AMP, glucose, glucose 6-phosphate, fructose 1,6-bisphosphate, fructose 6-phosphate, dihydroxyacetone phosphate, glyceraldehyde-3-phosphate, 3-phosphoglycerate, lactate, galactonate, gluconate, 2-keto-3-deoxygluconate, glucose 1-phosphate, citrate, isocitrate, alpha-ketoglutarate, succinate, fumarate, or malate | Thermoproteus tenax |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Thermoproteus tenax |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-glycerate | substrate inhibition is observed at higher glycerate concentrations | Thermoproteus tenax | |
ADP | 1 mM, 91.8% activity loss, at non-saturating concentrations of ATP, competitive inhibition | Thermoproteus tenax | |
EDTA | 40 mM | Thermoproteus tenax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
(R)-glycerate | at 70°C | Thermoproteus tenax | |
0.03 | - |
ATP | at 70°C | Thermoproteus tenax |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 20 mM, required for highest activity | Thermoproteus tenax | |
additional information | Co2+, Mn2+, and Ni2+ (all 5 mM), and Ca2+ (2 mM) can partially replace Mg2+ (56%, 59%, 30% and 15% activity, respectively) | Thermoproteus tenax | |
additional information | K+ and NH4+ do not stimulate activity | Thermoproteus tenax | |
additional information | no activity is detected in the presence of Cu2+ and Fe2+ at 0.2, 2, 5 and 20 mM | Thermoproteus tenax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42400 | - |
calculated from amino acid sequence | Thermoproteus tenax |
44000 | - |
SDS-PAGE, denatured enzyme | Thermoproteus tenax |
48300 | - |
native enzyme, HiLoad 26/60 Superdex 200 prep grade column gel filtration | Thermoproteus tenax |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoproteus tenax | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-sepharose column chromatography, phenyl sepharose column chromatography, and HiLoad 26/60 Superdex 200 prep grade gel filtration | Thermoproteus tenax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-glycerate | - |
Thermoproteus tenax | ADP + 3-phospho-(R)-glycerate | - |
? | |
additional information | ATP can be partially replaced by GTP, CTP, TTP and UTP (16%, 20%, 16%, and 10% activity, respectively) | Thermoproteus tenax | ? | - |
? | |
additional information | L-glycerate, galactonate, gluconate, malate, pyruvate, lactate, glyceraldehyde, glycerol, serine, 3-phosphoglycerate, ADP, diphosphate, polyphosphate, and glyceraldehyde 3-phosphate are no substrates | Thermoproteus tenax | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48300, gel filtration | Thermoproteus tenax |
Synonyms | Comment | Organism |
---|---|---|
More | member of the multi-organism fragment with rich leucine kinase family | Thermoproteus tenax |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermoproteus tenax |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
at 50°C 15% residual activity | Thermoproteus tenax |
70 | - |
at 70°C 55% residual activity | Thermoproteus tenax |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermoproteus tenax |