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Literature summary for 2.7.1.165 extracted from

  • Kehrer, D.; Ahmed, H.; Brinkmann, H.; Siebers, B.
    Glycerate kinase of the hyperthermophilic archaeon Thermoproteus tenax: new insights into the phylogenetic distribution and physiological role of members of the three different glycerate kinase classes (2007), BMC Genet., 8, 301.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information no increased activity in the presence of AMP, glucose, glucose 6-phosphate, fructose 1,6-bisphosphate, fructose 6-phosphate, dihydroxyacetone phosphate, glyceraldehyde-3-phosphate, 3-phosphoglycerate, lactate, galactonate, gluconate, 2-keto-3-deoxygluconate, glucose 1-phosphate, citrate, isocitrate, alpha-ketoglutarate, succinate, fumarate, or malate Thermoproteus tenax

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Thermoproteus tenax

Inhibitors

Inhibitors Comment Organism Structure
(R)-glycerate substrate inhibition is observed at higher glycerate concentrations Thermoproteus tenax
ADP 1 mM, 91.8% activity loss, at non-saturating concentrations of ATP, competitive inhibition Thermoproteus tenax
EDTA 40 mM Thermoproteus tenax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
(R)-glycerate at 70°C Thermoproteus tenax
0.03
-
ATP at 70°C Thermoproteus tenax

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 20 mM, required for highest activity Thermoproteus tenax
additional information Co2+, Mn2+, and Ni2+ (all 5 mM), and Ca2+ (2 mM) can partially replace Mg2+ (56%, 59%, 30% and 15% activity, respectively) Thermoproteus tenax
additional information K+ and NH4+ do not stimulate activity Thermoproteus tenax
additional information no activity is detected in the presence of Cu2+ and Fe2+ at 0.2, 2, 5 and 20 mM Thermoproteus tenax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42400
-
calculated from amino acid sequence Thermoproteus tenax
44000
-
SDS-PAGE, denatured enzyme Thermoproteus tenax
48300
-
native enzyme, HiLoad 26/60 Superdex 200 prep grade column gel filtration Thermoproteus tenax

Organism

Organism UniProt Comment Textmining
Thermoproteus tenax
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-sepharose column chromatography, phenyl sepharose column chromatography, and HiLoad 26/60 Superdex 200 prep grade gel filtration Thermoproteus tenax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-glycerate
-
Thermoproteus tenax ADP + 3-phospho-(R)-glycerate
-
?
additional information ATP can be partially replaced by GTP, CTP, TTP and UTP (16%, 20%, 16%, and 10% activity, respectively) Thermoproteus tenax ?
-
?
additional information L-glycerate, galactonate, gluconate, malate, pyruvate, lactate, glyceraldehyde, glycerol, serine, 3-phosphoglycerate, ADP, diphosphate, polyphosphate, and glyceraldehyde 3-phosphate are no substrates Thermoproteus tenax ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 48300, gel filtration Thermoproteus tenax

Synonyms

Synonyms Comment Organism
More member of the multi-organism fragment with rich leucine kinase family Thermoproteus tenax

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Thermoproteus tenax

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
at 50°C 15% residual activity Thermoproteus tenax
70
-
at 70°C 55% residual activity Thermoproteus tenax

Cofactor

Cofactor Comment Organism Structure
ATP
-
Thermoproteus tenax