Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
ATP | pH 8.0, 55°C | Thermoplasma acidophilum | |
0.32 | - |
ATP | in 100 mM Tris-HCl (pH 8), 5 mM EGTA, 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, at 55°C | Thermoplasma acidophilum | |
0.56 | - |
D-glycerate | pH 8.0, 55°C | Thermoplasma acidophilum | |
0.56 | - |
(R)-glycerate | in 100 mM Tris-HCl (pH 8), 5 mM EGTA, 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, at 55°C | Thermoplasma acidophilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM, can substitute for Mg2+, activity is 8% of the activity activated by Mg2+ | Thermoplasma acidophilum | |
Mg2+ | required | Thermoplasma acidophilum | |
Mg2+ | 10 mM, required for activity | Thermoplasma acidophilum | |
Mn2+ | 10 mM, can substitute for Mg2+, activity is 10% of the activity activated by Mg2+ | Thermoplasma acidophilum | |
additional information | when divalent metal ions, such as Mn2+, Co2+, Ni2+, Zn2+, Ca2+, and Sr2+, are substituted for Mg2+, the enzyme activity is less than 10% of that obtained in the presence of Mg2+ | Thermoplasma acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42992 | - |
1 * 42992, calculated from sequence | Thermoplasma acidophilum |
45000 | - |
SDS-PAGE, denatured enzyme | Thermoplasma acidophilum |
45000 | - |
1 * 45000, SDS-PAGE | Thermoplasma acidophilum |
45820 | - |
calculated from amino acid sequence | Thermoplasma acidophilum |
49000 | - |
SDS-PAGE, native enzyme | Thermoplasma acidophilum |
49300 | - |
gel filtration | Thermoplasma acidophilum |
49300 | - |
Sephacryl S-200 column gel filtration | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus P2 | - |
- |
- |
Thermoplasma acidophilum | - |
- |
- |
Thermoplasma acidophilum | Q9HKZ0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoplasma acidophilum |
DEAE Sepharose column chromatography, Q-Sepharose column chromatography, Phenyl-Sepharose column chromatography, and Affi-gel blue column chromatography | Thermoplasma acidophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
crude extract, using (R)-glycerate as a substrate, at pH 8.0 | Thermoplasma acidophilum |
118 | - |
after 747fold purification, using (R)-glycerate as a substrate, at pH 8.0 | Thermoplasma acidophilum |
118 | - |
pH 8.0, 55°C | Thermoplasma acidophilum |
Storage Stability | Organism |
---|---|
75°C, 100 mM Tris-HCl (pH 8), over 2 h, no loss of activity | Thermoplasma acidophilum |
78°C, 100 mM Tris-HCl (pH 8), 1 h, 50% loss of activity | Thermoplasma acidophilum |
80°C, 100 mM Tris-HCl (pH 8), 20 min, 95% loss of activity | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-glycerate | - |
Thermoplasma acidophilum | ADP + 3-phospho-(R)-glycerate | - |
? | |
ATP + D-glycerate | - |
Saccharolobus solfataricus | ADP + 2-phospho-D-glycerate | - |
? | |
ATP + D-glycerate | no activity with DL-glyceraldehyde, 2-phospho-D-glycerate, 3-phospho-D-glycerate or L-tartrate. Activity with D-gluconate or glycerol at 5% of the activity compared to D-glycerate. Specificity for ATP as a phosphate donor. GTP, CTP, and UTP exhibit 0~4% of the activity of ATP | Thermoplasma acidophilum | ADP + 2-phospho-D-glycerate | - |
? | |
ATP + D-glycerate | - |
Saccharolobus solfataricus P2 | ADP + 2-phospho-D-glycerate | - |
? | |
additional information | GTP, CTP, UTP, D-glucose, D-gluconate, glycerol, D-fructose, DL-glyceraldehyde, D-ribose, D-xylose, 2-phospho-D-glycerate, 3-phospho-D-glycerate, and L-tartrate are no substrates | Thermoplasma acidophilum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Thermoplasma acidophilum |
monomer | 1 * 49000, SDS-PAGE, native enzyme | Thermoplasma acidophilum |
monomer | 1 * 42992, calculated from sequence | Thermoplasma acidophilum |
Synonyms | Comment | Organism |
---|---|---|
Ta0453 | locus name | Thermoplasma acidophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Thermoplasma acidophilum |
70 | - |
- |
Thermoplasma acidophilum |
75 | - |
assay at | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 85 | 20°C: about 40% of maximal activity, 85°C: about 50% of maximal activity | Thermoplasma acidophilum |
70 | 80 | activity decreases rapidly at temperatures above 80°C, but slowly at temperatures below 70°C | Thermoplasma acidophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | exhibits excellent thermal stability at 70°C, activity decreases rapidly at temperatures above 80°C, but slowly at temperatures below 70°C, at temperatures up to 75°C, glycerate kinase is stable for over 2 h but at 78°C its activity is reduced to less than 50% in one hour, and at 80°C its activity diminishes rapidly, with less than 5% of its initial activity remaining after 20 min | Thermoplasma acidophilum |
75 | - |
stable for over 2 h | Thermoplasma acidophilum |
78 | - |
1 h, activity is reduced to less than 50% | Thermoplasma acidophilum |
80 | - |
less than 5% of its initial activity remaining after 20 min | Thermoplasma acidophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharolobus solfataricus |
8 | - |
assay at | Thermoplasma acidophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermoplasma acidophilum |