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Literature summary for 2.7.1.165 extracted from

  • Noh, M.; Jung, J.H.; Lee, S.B.
    Purification and characterization of glycerate kinase from the thermoacidophilic archaeon Thermoplasma acidophilum: an enzyme belonging to the second glycerate kinase family (2006), Biotechnol. Bioprocess Eng., 11, 344-350.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
ATP pH 8.0, 55°C Thermoplasma acidophilum
0.32
-
ATP in 100 mM Tris-HCl (pH 8), 5 mM EGTA, 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, at 55°C Thermoplasma acidophilum
0.56
-
D-glycerate pH 8.0, 55°C Thermoplasma acidophilum
0.56
-
(R)-glycerate in 100 mM Tris-HCl (pH 8), 5 mM EGTA, 1 mM (R)-glycerate, 1 mM ATP, 10 mM MgCl2, at 55°C Thermoplasma acidophilum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 10 mM, can substitute for Mg2+, activity is 8% of the activity activated by Mg2+ Thermoplasma acidophilum
Mg2+ required Thermoplasma acidophilum
Mg2+ 10 mM, required for activity Thermoplasma acidophilum
Mn2+ 10 mM, can substitute for Mg2+, activity is 10% of the activity activated by Mg2+ Thermoplasma acidophilum
additional information when divalent metal ions, such as Mn2+, Co2+, Ni2+, Zn2+, Ca2+, and Sr2+, are substituted for Mg2+, the enzyme activity is less than 10% of that obtained in the presence of Mg2+ Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42992
-
1 * 42992, calculated from sequence Thermoplasma acidophilum
45000
-
SDS-PAGE, denatured enzyme Thermoplasma acidophilum
45000
-
1 * 45000, SDS-PAGE Thermoplasma acidophilum
45820
-
calculated from amino acid sequence Thermoplasma acidophilum
49000
-
SDS-PAGE, native enzyme Thermoplasma acidophilum
49300
-
gel filtration Thermoplasma acidophilum
49300
-
Sephacryl S-200 column gel filtration Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus P2
-
-
-
Thermoplasma acidophilum
-
-
-
Thermoplasma acidophilum Q9HKZ0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoplasma acidophilum
DEAE Sepharose column chromatography, Q-Sepharose column chromatography, Phenyl-Sepharose column chromatography, and Affi-gel blue column chromatography Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
crude extract, using (R)-glycerate as a substrate, at pH 8.0 Thermoplasma acidophilum
118
-
after 747fold purification, using (R)-glycerate as a substrate, at pH 8.0 Thermoplasma acidophilum
118
-
pH 8.0, 55°C Thermoplasma acidophilum

Storage Stability

Storage Stability Organism
75°C, 100 mM Tris-HCl (pH 8), over 2 h, no loss of activity Thermoplasma acidophilum
78°C, 100 mM Tris-HCl (pH 8), 1 h, 50% loss of activity Thermoplasma acidophilum
80°C, 100 mM Tris-HCl (pH 8), 20 min, 95% loss of activity Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-glycerate
-
Thermoplasma acidophilum ADP + 3-phospho-(R)-glycerate
-
?
ATP + D-glycerate
-
Saccharolobus solfataricus ADP + 2-phospho-D-glycerate
-
?
ATP + D-glycerate no activity with DL-glyceraldehyde, 2-phospho-D-glycerate, 3-phospho-D-glycerate or L-tartrate. Activity with D-gluconate or glycerol at 5% of the activity compared to D-glycerate. Specificity for ATP as a phosphate donor. GTP, CTP, and UTP exhibit 0~4% of the activity of ATP Thermoplasma acidophilum ADP + 2-phospho-D-glycerate
-
?
ATP + D-glycerate
-
Saccharolobus solfataricus P2 ADP + 2-phospho-D-glycerate
-
?
additional information GTP, CTP, UTP, D-glucose, D-gluconate, glycerol, D-fructose, DL-glyceraldehyde, D-ribose, D-xylose, 2-phospho-D-glycerate, 3-phospho-D-glycerate, and L-tartrate are no substrates Thermoplasma acidophilum ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Thermoplasma acidophilum
monomer 1 * 49000, SDS-PAGE, native enzyme Thermoplasma acidophilum
monomer 1 * 42992, calculated from sequence Thermoplasma acidophilum

Synonyms

Synonyms Comment Organism
Ta0453 locus name Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
assay at Thermoplasma acidophilum
70
-
-
Thermoplasma acidophilum
75
-
assay at Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 85 20°C: about 40% of maximal activity, 85°C: about 50% of maximal activity Thermoplasma acidophilum
70 80 activity decreases rapidly at temperatures above 80°C, but slowly at temperatures below 70°C Thermoplasma acidophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 80 exhibits excellent thermal stability at 70°C, activity decreases rapidly at temperatures above 80°C, but slowly at temperatures below 70°C, at temperatures up to 75°C, glycerate kinase is stable for over 2 h but at 78°C its activity is reduced to less than 50% in one hour, and at 80°C its activity diminishes rapidly, with less than 5% of its initial activity remaining after 20 min Thermoplasma acidophilum
75
-
stable for over 2 h Thermoplasma acidophilum
78
-
1 h, activity is reduced to less than 50% Thermoplasma acidophilum
80
-
less than 5% of its initial activity remaining after 20 min Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Saccharolobus solfataricus
8
-
assay at Thermoplasma acidophilum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Thermoplasma acidophilum