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Literature summary for 2.7.1.164 extracted from
- Leishmania donovani encodes a functional selenocysteinyl-tRNA synthase (2016), J. Biol. Chem., 291, 1203-1220.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Leishmania donovani |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-seryl-tRNASec | Leishmania donovani | - |
ADP + O-phospho-L-seryl-tRNASec | - |
? | |
| ATP + L-seryl-tRNASec | Leishmania donovani Bob | - |
ADP + O-phospho-L-seryl-tRNASec | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | - |
gene PSTK | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-seryl-tRNASec | - |
Leishmania donovani | ADP + O-phospho-L-seryl-tRNASec | - |
? | |
| ATP + L-seryl-tRNASec | - |
Leishmania donovani Bob | ADP + O-phospho-L-seryl-tRNASec | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| O-phosphoseryl-tRNA kinase | - |
Leishmania donovani |
| PSTK | - |
Leishmania donovani |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Leishmania donovani | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the synthesis of selenocysteine, the 21st amino acid, occurs on its transfer RNA, tRNASec. tRNASec is initially aminoacylated with serine by seryl-tRNA synthetase and the resulting seryl moiety is converted to phosphoserine by O-phosphoseryl-tRNA kinase (PSTK) in eukaryotes. The selenium donor, selenophosphate is synthesized from selenide and ATP by selenophosphate synthetase. Selenocysteinyl-tRNA synthase (Sep-SecS) then uses the O-phosphoseryl-tRNASec and selenophosphate to form Sec-tRNASec in eukaryotes. Leishmania SepSecS enzyme is active and able to complement the DELTAselA deletion in Escherichia coli JS1 strain only in the presence of archaeal PSTK, indicating the conserved nature of the PSTK-SepSecS pathway, selenocysteinyl-tRNA synthase is dependent on the action of PSTK enzyme in the Sec insertion pathway | Leishmania donovani |
| physiological function | the enzyme is essential in the selenocysteine insertion | Leishmania donovani |
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Release 2023.1 (January 2023)
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