Literature summary for 2.7.1.163 extracted from

Sripriya, R.; Raghupathy, V.; Veluthambi, K.
Generation of selectable marker-free sheath blight resistant transgenic rice plants by efficient co-transformation of a cointegrate vector T-DNA and a binary vector T-DNA in one Agrobacterium tumefaciens strain (2008), Plant Cell Rep., 27, 1635-1644.

Search for more literature for 2.7.1.163

Application

Application	Comment	Organism
biotechnology	resistance against hyromycin B mediated by transformation of the hph gene	Escherichia coli
molecular biology	selectable marker (SM) genes, essential to select transformed cells from a large population of untransformed cells	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
Co-transformation of Oryza sativa L. var. Pusa Basmati1 was done using an Agrobacterium tumefaciens strain harbouring a single-copy cointegrate vector and a multi-copy binary vector in the same cell. The T-DNA of the cointegrate plasmid pGV2260::pSSJ1 carried the hygromycin phosphotransferase and beta-glucuronidase genes. The binary vector pCam-chi11, without a plant selectable marker gene, harboured the rice chitinase gene under maize ubiquitin promoter.	Escherichia coli

Cloned (Comment)

Organism

Co-transformation of Oryza sativa L. var. Pusa Basmati1 was done using an Agrobacterium tumefaciens strain harbouring a single-copy cointegrate vector and a multi-copy binary vector in the same cell. The T-DNA of the cointegrate plasmid pGV2260::pSSJ1 carried the hygromycin phosphotransferase and beta-glucuronidase genes. The binary vector pCam-chi11, without a plant selectable marker gene, harboured the rice chitinase gene under maize ubiquitin promoter.

Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	Agrobacterium tumefaciens strain C58C1 Rif-R harbouring a cointegrate plasmid pGV2260::pSSJ1 and a binary plasmid pCam-chi11 is used for co-transformation in rice. The T-DNA of the cointegrate vector pGV2260::pSSJ1 carries hph gene from Escherichia coli.	-

Synonyms

Synonyms	Comment	Organism
HPH	-	Escherichia coli
hygromycin phosphotransferase	-	Escherichia coli

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Release 2023.1 (January 2023)