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Literature summary for 2.7.1.163 extracted from
- Regeneration of transgenic plants from two indica rice (Oryza sativa L.) cultivars using shoot apex explants (2007), Plant Cell Rep., 26, 1745-1753.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | as selective marker gene | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Reproducible procedure for transformation of shoot apices and regeneration of transgenic plants for two indica rice cultivars. Shoot apex explants are transformed by cocultivation with Agrobacterium tumefaciens strain EHA 101 harbouring the binary plasmid pRIT1. Vector contains an improved hygromycin phosphotransferase gene for hygromycin resistance driven by actin 1 promoter and the reporter gene beta-glucuronidase intron controlled by CaMV 35S promoter. | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
Agrobacterium tumefaciens EHA101 (pRIT1) is used for transformation and the vector pRIT1 has been described previously. T-DNA of pRIT 1 carries the highly active rice Actin 1 promoter with its intron 1 fused with an hpt gene modified at its 3' coding region and the cauliflower virus. | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | strain EHA 101 is used for transformation | Escherichia coli | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HPT | - |
Escherichia coli |
| hygromycin phosphotransferase | - |
Escherichia coli |
| HyR | - |
Escherichia coli |
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