Literature summary for 2.7.1.163 extracted from

Ishizaki, K.; Chiyoda, S.; Yamato, K.T.; Kohchi, T.
Agrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology (2008), Plant Cell Physiol., 49, 1084-1091.

Search for more literature for 2.7.1.163

Application

Application	Comment	Organism
biotechnology	resistance against hyromycin B mediated by transformation of the hph gene	Escherichia coli
molecular biology	Used as marker gene. Mediates hygromycin resistance.	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
by Agrobacterium-mediated transformation. Spores of Marchantia polymorpha are germinated and grown into immature thalli. The 7-day-old immature thalli are co-cultivated with Agrobacterium and transferred directly to selective M51C agar medium after washing. Incubation of immature thalli with Agrobacterium harboring the binary plasmid pIG121Hm lead to the formation of hygromycin-resistant plantlets, whereas Agrobacterium carrying no binary plasmid did not. Hygromycin-resistant thalli with rhizoids became distinct 10 days after transfer to the selection agar medium, whereas hygromycin-sensitive plantlets developed into chlorotic cell clumps. To avoid chimerism of hygromycin-resistant thalli, isogenic lines are obtained from gemmae which arise asexually from single initial cells in cupules, and used for further analysis.	Escherichia coli

Cloned (Comment)

Organism

by Agrobacterium-mediated transformation. Spores of Marchantia polymorpha are germinated and grown into immature thalli. The 7-day-old immature thalli are co-cultivated with Agrobacterium and transferred directly to selective M51C agar medium after washing. Incubation of immature thalli with Agrobacterium harboring the binary plasmid pIG121Hm lead to the formation of hygromycin-resistant plantlets, whereas Agrobacterium carrying no binary plasmid did not. Hygromycin-resistant thalli with rhizoids became distinct 10 days after transfer to the selection agar medium, whereas hygromycin-sensitive plantlets developed into chlorotic cell clumps. To avoid chimerism of hygromycin-resistant thalli, isogenic lines are obtained from gemmae which arise asexually from single initial cells in cupules, and used for further analysis.

Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	binary plasmid pIG121Hm containing hpt gene is transformed into Marchantia polymorpha via Agrobacterium tumefaciens (strain GV2260)	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Escherichia coli	-
additional information	Immature thalli of Marchantia developing from spores are used for Agrobacterium tumefaciens infection	Escherichia coli	-

Synonyms

Synonyms	Comment	Organism
HPT	-	Escherichia coli
hygromycin phosphotransferase	-	Escherichia coli