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Literature summary for 2.7.1.163 extracted from
- Agrobacterium tumefaciens-mediated transformation in Colletotrichum falcatum and C. acutatum (2008), J. Microbiol. Biotechnol., 18, 234-241.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | resistance against hyromycin B mediated by transformation of the hph gene | Escherichia coli |
| molecular biology | resistance against hyromycin B mediated by transformation of the hph gene, a selectable marker gen. | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in Colletotrichum falcatum and Colletotrichum acutatum. Agrobacterium tumefaciens-mediated transformation (ATMT) is used by using the vector pBHt2 that contains a t-DNA harboring the hygromycin B resistance gen (hygromycin phosphotransferase, hph) in the backbone of pCAMBIA1300. | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hygromycin B + ATP | Escherichia coli | Growth of Colletotrichum falcatum is completely inhibited at 50 microg/ml, whereas in Colletotrichum acutatum, a concentration of 300 microg/ml is most effective in inhibiting mycelial growth | 4-O-phosphohygromycin B + ADP | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
The vector pBHt2 contains a T-DNA harboring hygromycin B resistance (hygromycin phosphotransferase, hph) in the backbone of pCAMBIA1300 descended from Escherichia coli, transformed in Colletrichum acutatum and Colletrichum falcatum via Agrobacterium tumefaciens | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | bacterial strain SK1044 | Escherichia coli | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hygromycin B + ATP | Growth of Colletotrichum falcatum is completely inhibited at 50 microg/ml, whereas in Colletotrichum acutatum, a concentration of 300 microg/ml is most effective in inhibiting mycelial growth | Escherichia coli | 4-O-phosphohygromycin B + ADP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HPH | - |
Escherichia coli |
| hygromycin phosphotransferase | - |
Escherichia coli |
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