Application | Comment | Organism |
---|---|---|
biotechnology | the enzyme is widely used as selective marker gene product in production of engineered crops, e.g. rice | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
subcloning in strain DH5alpha, overexpression of non-tagged enzyme in strain BL21(DE3) in inclusion bodies, method optimization, expression in transgenic rice plants | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic Oryza sativa plants expressing the enzyme in leaves, roots, and seeds | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | at 20 mM | Escherichia coli | |
Mg2+ | at 10 mM MgCl2 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, recombinant enzyme from transgenic rice plants, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + hygromycin B | Escherichia coli | - |
ADP + 7''-O-phosphohygromycin B | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
soluble renatured recombinant enzyme by anion exchange chromatography to over 95% purity | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme from inclusion bodies after overexpression in Escherichia coli, solubilization by 0.3% sarcosine, renaturation by dilution and dialysis, method optimization | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8 | - |
purified refolded recombinant enzyme expressed in Escherichia coli | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + hygromycin B | - |
Escherichia coli | ADP + 7''-O-phosphohygromycin B | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, recombinant enzyme from transgenic rice plants, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
HPT | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |