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Literature summary for 2.7.1.163 extracted from

  • Zhuo, Q.; Piao, J.H.; Wang, R.; Yang, X.G.
    Refolding and purification of non-fusion HPT protein expressed in Escherichia coli as inclusion bodies (2005), Protein Expr. Purif., 41, 53-60.
    View publication on PubMed

Application

Application Comment Organism
biotechnology the enzyme is widely used as selective marker gene product in production of engineered crops, e.g. rice Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
subcloning in strain DH5alpha, overexpression of non-tagged enzyme in strain BL21(DE3) in inclusion bodies, method optimization, expression in transgenic rice plants Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of transgenic Oryza sativa plants expressing the enzyme in leaves, roots, and seeds Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
KCl at 20 mM Escherichia coli
Mg2+ at 10 mM MgCl2 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
x * 38000, recombinant enzyme from transgenic rice plants, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + hygromycin B Escherichia coli
-
ADP + 7''-O-phosphohygromycin B
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
soluble renatured recombinant enzyme by anion exchange chromatography to over 95% purity Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
recombinant enzyme from inclusion bodies after overexpression in Escherichia coli, solubilization by 0.3% sarcosine, renaturation by dilution and dialysis, method optimization Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8
-
purified refolded recombinant enzyme expressed in Escherichia coli Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + hygromycin B
-
Escherichia coli ADP + 7''-O-phosphohygromycin B
-
?

Subunits

Subunits Comment Organism
? x * 38000, recombinant enzyme from transgenic rice plants, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
HPT
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli