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Literature summary for 2.7.1.163 extracted from

  • Yang, L.C.; Zhu, Z.; Yang, X.G.
    Purification and immunity analysis of recombinant 6His-HPT protein expressed in E. coli (2003), Biomed. Environ. Sci., 16, 149-156.
    View publication on PubMed

Application

Application Comment Organism
biotechnology the enzyme can be used as selective marker gene product in production of transgenic plants Escherichia coli
molecular biology the enzyme can be used as selective marker gene product in production of transgenic plants Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, overexpression of His-tagged enzyme in strain DH5alpha in inclusion bodies Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme denatured by 8 M urea, by nickel affinity chromatography to over 95% purity showing high immunoactivity Escherichia coli

Synonyms

Synonyms Comment Organism
HPT
-
Escherichia coli
hygromycin B phosphotransferase
-
Escherichia coli