Literature summary for 2.7.1.163 extracted from

Zalacain, M.; Malpartida, F.; Pulido, D.; Jimenez, A.
Cloning and expression in Escherichia coli of a hygromycin B phosphotransferase gene from Streptomyces hygroscopicus (1987), Eur. J. Biochem., 162, 413-418.

Search for more literature for 2.7.1.163

Cloned(Commentary)

Cloned (Comment)	Organism
hyg-gene introduced into different sites of both the Escherichia coli plasmid pBR322 and the Escherichia coli-Saccharomyces cerevisiae shuttle vector YRp7. When this gene is inserted into the BamHI site of pBR322 and then cloned in Escherichia coli phosphorylating activity is not detected. When the hyg gene is inserted into either the unique PstI site of the pBR322 or into each of the two PstI sites of YRp7, phosphotransferase activity is observed	Streptomyces hygroscopicus

Cloned (Comment)

Organism

hyg-gene introduced into different sites of both the Escherichia coli plasmid pBR322 and the Escherichia coli-Saccharomyces cerevisiae shuttle vector YRp7. When this gene is inserted into the BamHI site of pBR322 and then cloned in Escherichia coli phosphorylating activity is not detected. When the hyg gene is inserted into either the unique PstI site of the pBR322 or into each of the two PstI sites of YRp7, phosphotransferase activity is observed

Streptomyces hygroscopicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Streptomyces hygroscopicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42000	-	non-dentauring PAGE	Streptomyces hygroscopicus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces hygroscopicus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + hygromycin B	-	Streptomyces hygroscopicus	ADP + 7''-O-phosphohygromycin B	-	?

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Release 2023.1 (January 2023)