Application | Comment | Organism |
---|---|---|
additional information | Inhibitors of Tpt1p might prove useful as selective antifungal agents. | Mus musculus |
Cloned (Comment) | Organism |
---|---|
The murine Trpt1 gene is targeted in E14 ES cells with a positive/negative selection vector. Trpt1 gene is disrupted by homologous recombination in ES cells, deleting exons 3, 4, and part of exon 5 that encode amino acids 48-173. Clones from two individually selected heterozygous ES cell lines are injected into blastocysts and the resultant founder chimeras bred to obtain two lines of Trpt1+/- and Trpt1-/-mice that are both viable and healthy and are normally fertile. Cell culture: Trpt1+/+ and Trpt1-/-cell lines are obtained by serial passage of SV40-T-antigen transfected mouse embryonic fibroblasts. | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM in the activity assay | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-phospho-(ligated tRNA) + NAD+ | Mus musculus | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide + H+ | - |
? | |
additional information | Mus musculus | Comparison of incorporation of 3H-tyrosine and 35S-methionine/cysteine into total proteins confirms that wild-type and Trpt1-/-cells have similar incorporation rates of either amino acid. | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
inactivation of the enzyme is done in knockout mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blastocyst | Clones from two individually selected heterozygous ES cell lines are injected into blastocysts and the resultant founder chimeras bred to obtain two lines of knockout Trpt1+/- and knockout Trpt1-/-mice. | Mus musculus | - |
heart | - |
Mus musculus | - |
MEF cell | Trpt1+/+ and Trpt1-/-cell lines are obtained by serial passage of SV40-T-antigen transfected mouse embryonic fibroblasts | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Despite lack of detectable in vitro RNA 2'-phosphotransferase activity in lysates of cells derived from the Trpt1 knockout mice, there is no evidence for a defect in the unfolded protein response or in tRNA function. | Mus musculus |
additional information | - |
Immunoprecipitation of Trpt1 from heart lysates, followed by immunoblot with an antiserum raised to bacterially expressed murine Trpt1 reveals that Trpt1-/-mice lack 2'-phosphotransferase activity yet retain the ability to translate spliced transcription factor XBP-1 mRNA and synthesize tyrosine-containing proteins at wild-type levels. Heterozygotes express 50% of wild-type levels. Extracts of Trpt1+/+cells have measurable NAD+-dependent 2'-phosphotransferase activity. | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-phospho-(ligated tRNA) + NAD+ | - |
Mus musculus | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide + H+ | - |
? | |
additional information | Comparison of incorporation of 3H-tyrosine and 35S-methionine/cysteine into total proteins confirms that wild-type and Trpt1-/-cells have similar incorporation rates of either amino acid. | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2'-phosphotransferase | - |
Mus musculus |
Tpt1p | Mammalian genomes encode a single known functional homolog of yeast Tpt1p. | Mus musculus |
Trpt1 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mus musculus |