Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.160 extracted from

  • Steiger, M.A.; Jackman, J.E.; Phizicky, E.M.
    Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism (2005), RNA, 11, 99-106.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C85R no accumulation of the reaction intermediate Saccharomyces cerevisiae
D34A no accumulation of the reaction intermediate Saccharomyces cerevisiae
H117A no accumulation of the reaction intermediate Saccharomyces cerevisiae
H140A no accumulation of the reaction intermediate Saccharomyces cerevisiae
H140A/H142A no accumulation of the reaction intermediate Saccharomyces cerevisiae
H140A/V141A/H142A no accumulation of the reaction intermediate Saccharomyces cerevisiae
H142A no accumulation of the reaction intermediate Saccharomyces cerevisiae
H90A no accumulation of the reaction intermediate Saccharomyces cerevisiae
I167L/D168K no accumulation of the reaction intermediate Saccharomyces cerevisiae
K69A/R71S this mutant produces a substantial amount of the reaction intermediate Saccharomyces cerevisiae
M136G no accumulation of the reaction intermediate Saccharomyces cerevisiae
N185A no accumulation of the reaction intermediate Saccharomyces cerevisiae
Q88A/H90A/S91A no accumulation of the reaction intermediate Saccharomyces cerevisiae
Q88Y no accumulation of the reaction intermediate Saccharomyces cerevisiae
R138A no accumulation of the reaction intermediate Saccharomyces cerevisiae
R158H no accumulation of the reaction intermediate Saccharomyces cerevisiae
R23A/H24A no accumulation of the reaction intermediate Saccharomyces cerevisiae
S155A no accumulation of the reaction intermediate Saccharomyces cerevisiae
S15A/K16A no accumulation of the reaction intermediate Saccharomyces cerevisiae
S183A no accumulation of the reaction intermediate Saccharomyces cerevisiae
S91A no accumulation of the reaction intermediate Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography, > 80% purified by SDS-PAGE Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + trinucleotide substrate requires ca. 0.0004 mM Tpt1 K69A/R71S, only 0.0000003 mM Tpt1p is required for similar or greater product formation Saccharomyces cerevisiae ADP-ribosylated RNA
-
?

Synonyms

Synonyms Comment Organism
Tpt1p
-
Saccharomyces cerevisiae