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Literature summary for 2.7.1.160 extracted from

  • Zillman, M.; Gorovsky, M.A.; Phizicky, E.M.
    HeLa cells contain a 2'-phosphate-specific phosphotransferase similar to a yeast enzyme implicated in tRNA splicing (1992), J. Biol. Chem., 267, 10289-10294.
    View publication on PubMed
Search for more literature for 2.7.1.160

Organism

Organism UniProt Comment Textmining
Homo sapiens
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Saccharomyces cerevisiae
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Purification (Commentary)

Purification (Comment) Organism
partially purified by chromatography on heparin-agarose Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
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 Homo sapiens
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Storage Stability

Storage Stability Organism
-20, Tris-HCl buffer, pH. 8.0, 50% glycerol, several months Homo sapiens
-80°C, Tris-HCl buffer, pH 8.0, 20% glycerol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-phospho-[ligated tRNA] + NAD+ minimal substrate is a dinucleotide containing an internal 2' phosphate, 600fold more specific for 2'phosphates than for eihter 5'- or 3'-terminal phosphates, 20fold more specific for internal 2' phosphates than for terminal 2' 3', or 2'3'-cyclic phosphates, at least 50fold more specific for internal 2' phosphates than terminal 2' phosphates Homo sapiens mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
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 ?
2'-phospho-[ligated tRNA] + NAD+ minimal substrate is a dinucleotide containing an internal 2'-phosphate Saccharomyces cerevisiae mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
HeLa cell 2'-phosphotransferase
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 Homo sapiens
yeast 2'-phosphotransferase
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 Saccharomyces cerevisiae