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Literature summary for 2.7.1.16 extracted from
- Crystalline L-ribulokinase from Escherichia coli (1967), J. Biol. Chem., 242, 2043-2050.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.076 | - |
ATP | 37°C, pH 7.6 | Escherichia coli |  |
| 0.111 | - |
L-ribulose | 37°C, pH 7.6 | Escherichia coli | |
| 0.27 | - |
D-ribulose | 37°C, pH 7.6 | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Escherichia coli | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement, Km-value: 1.3 mM | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 96500 | 100000 | osmotic pressure measurement, ultracentrifugation, ultracentrifugation and diffusion method | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribulose | Escherichia coli | - |
ADP + D-ribulose 5-phosphate | - |
? | |
| ATP + D-ribulose | Escherichia coli bB/r | - |
ADP + D-ribulose 5-phosphate | - |
? | |
| ATP + L-ribulose | Escherichia coli | inducible enzyme of L-arabinose catabolism | ADP + L-ribulose 5-phosphate | - |
? | |
| ATP + L-ribulose | Escherichia coli bB/r | inducible enzyme of L-arabinose catabolism | ADP + L-ribulose 5-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
mutant araA-2 | - |
| Escherichia coli bB/r | - |
mutant araA-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 833 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribulose | - |
Escherichia coli | ADP + D-ribulose 5-phosphate | - |
? | |
| ATP + D-ribulose | - |
Escherichia coli bB/r | ADP + D-ribulose 5-phosphate | - |
? | |
| ATP + L-ribulose | no substrates are both isomers of xylulose and other aldopentoses | Escherichia coli | ADP + L-ribulose 5-phosphate | - |
? | |
| ATP + L-ribulose | inducible enzyme of L-arabinose catabolism | Escherichia coli | ADP + L-ribulose 5-phosphate | - |
? | |
| ATP + L-ribulose | no substrates are both isomers of xylulose and other aldopentoses | Escherichia coli bB/r | ADP + L-ribulose 5-phosphate | - |
? | |
| ATP + L-ribulose | inducible enzyme of L-arabinose catabolism | Escherichia coli bB/r | ADP + L-ribulose 5-phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60.5 | - |
at least 30 min stable at pH 7.6 | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
and above, stable at least 30 min at 60.5°C | Escherichia coli |
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Release 2023.1 (January 2023)
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