Cloned (Comment) | Organism |
---|---|
gene ITPK2, expression analysis of wild-type and mutant enzymes by quantitative real-time PCR analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | functional analysis of itpk2-1 mutant (SAIL_25_H09), phenotype, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Arabidopsis thaliana | 5618 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,3,4-trisphosphate | Arabidopsis thaliana | - |
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,3,4-trisphosphate | Arabidopsis thaliana | - |
ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O81893 | gene ITPK2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | very low expression level | Arabidopsis thaliana | - |
leaf | very low expression level | Arabidopsis thaliana | - |
additional information | expression pattern of isozyme ITPK2, no or very poor expression in stem | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
seed | - |
Arabidopsis thaliana | - |
seedling | very low expression level | Arabidopsis thaliana | - |
silique | very low expression level | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,3,4-trisphosphate | - |
Arabidopsis thaliana | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,3,4-trisphosphate | - |
Arabidopsis thaliana | ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate | - |
? | |
additional information | the enzyme also performs the reaction of EC 2.7.1.134, phosphorylation of position 1 of 1D-myo-inositol 3,4,5,6-tetrakisphosphate | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ITPK2 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | the epidermis structure of seed coat is irregularly formed in seeds of itpk2-1 mutant, resulting in the increased permeability of seed coat to tetrazolium salts. The cell wall shows a dramatic decrease in composition of suberin and cutin, which relate to the permeability of seed coat and the formation of which is accompanied with seed coat development. ITPK2 deficiency results in the distorted seed coat and crumpled columellas. Seed coat of itpk2-1 mutant presents high permeability to 2,3,5-triphenyltetrazolium and has less mucilage | Arabidopsis thaliana |
physiological function | enzyme ITPK2 plays an essential role in seed coat development and lipid polyester barrier formation | Arabidopsis thaliana |