Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.159 extracted from

  • Field, J.; Wilson, M.P.; Mai, Z.; Majerus, P.W.; Samuelson, J.
    An Entamoeba histolytica inositol 1,3,4-trisphosphate 5/6-kinase has a novel 3-kinase activity (2000), Mol. Biochem. Parasitol., 108, 119-123.
    View publication on PubMed
Search for more literature for 2.7.1.159

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme expression is slightly induced by heat shock Entamoeba histolytica

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression as GST-tagged enzyme in bacteria Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol-1,3,4-trisphosphate Entamoeba histolytica
-
 ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate Entamoeba histolytica
-
 ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate Entamoeba histolytica HM-1
-
 ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate Entamoeba histolytica HM-1
-
 ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica Q9XYQ1
-
-
Entamoeba histolytica HM-1 Q9XYQ1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme, the GST-tag is cleaved off by treatment with GST-3Cpro Entamoeba histolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate the formation of 1D-myo-inositol-1,3,4,6-tetrakisphosphate is slightly preferred compared to formation of 1D-myo-inositol-1,3,4,5-tetrakisphosphate, ratio 1.5:1 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica HM-1 ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica HM-1 ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate the formation of 1D-myo-inositol-1,3,4,6-tetrakisphosphate is slightly preferred compared to formation of 1D-myo-inositol-1,3,4,5-tetrakisphosphate, ratio 1.5:1 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate the formation of 1D-myo-inositol-1,3,4,6-tetrakisphosphate is slightly preferred compared to formation of 1D-myo-inositol-1,3,4,5-tetrakisphosphate, ratio 1.5:1 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica HM-1 ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
 Entamoeba histolytica HM-1 ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate the formation of 1D-myo-inositol-1,3,4,6-tetrakisphosphate is slightly preferred compared to formation of 1D-myo-inositol-1,3,4,5-tetrakisphosphate, ratio 1.5:1 ?
additional information the enzyme also preferably performs the phosphoinositol trisphosphate 3-kinase reaction with 1D-myo-inositol-1,4,5-trisphosphate as substrate, EC 2.7.1.127, no activity with 1D-myo-inositol-1,4-bisphosphate Entamoeba histolytica ?
-
 ?
additional information the enzyme also preferably performs the phosphoinositol trisphosphate 3-kinase reaction with 1D-myo-inositol-1,4,5-trisphosphate as substrate, EC 2.7.1.127, no activity with 1D-myo-inositol-1,4-bisphosphate Entamoeba histolytica HM-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
Ins(1,3,4)P3 5/6-kinase
-
 Entamoeba histolytica

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Entamoeba histolytica