Any feedback?
Literature summary for 2.7.1.159 extracted from
- Inositol 1,3,4-trisphosphate 5/6-kinase is a protein kinase that phosphorylates the transcription factors c-Jun and ATF-2 (2001), J. Biol. Chem., 276, 40998-41004.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in 293 cells, expression in Spodoptera frugiperda Sf21 cells using the baculovirus infection system | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1D-myo-inositol-3,4,5,6-tetrakisphosphate | inhibits the 5/6-kinase activities, but not the phosphorylation of ATF-2 | Bos taurus |  |
| Mn2+ | activates the protein phopshorylation, but inhibits the inositol-1,3,4-trisphosphate 5/6-kinase reaction | Bos taurus | |
| protein kinase A | slight inhibition of enzyme activity with ATF-2 as substrate | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
| Mg2+ | - |
Bos taurus | |
| Mn2+ | activates the protein phopshorylation, but inhibits the inositol-1,3,4-trisphosphate 5/6-kinase reaction | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | Bos taurus | - |
ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | Bos taurus | - |
ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate | - |
? | |
| additional information | Bos taurus | the enzyme is involved in both the inositol phosphate and the stress response pathways which are linked by the enzyme, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially as COP9 signalosome complex and further as a single protein | Bos taurus |
| recombinant enzyme from insect Sf21 cells, recombinant enzyme partially from 293 cells by ion exchange chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 372 | - |
purified calf brain enzyme, substrate 1D-myo-inositol-1,3,4-trisphosphate | Bos taurus |
| 500 | - |
purified recombinant enzyme from Sf21 cells, with substrate 1D-myo-inositol-1,3,4-trisphosphate | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | - |
Bos taurus | ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | 5fold lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation | Homo sapiens | ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | 5fold lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation | Bos taurus | ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | - |
Bos taurus | ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | 5fold higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation | Homo sapiens | ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | 5fold higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation | Bos taurus | ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate | - |
? | |
| ATP + ATF-2 | phosphorylation predominantly on serine residues | Homo sapiens | ADP + phosphorylated ATF-2 | - |
? | |
| ATP + ATF-2 | phosphorylation predominantly on serine residues | Bos taurus | ADP + phosphorylated ATF-2 | - |
? | |
| ATP + c-Jun | - |
Homo sapiens | ADP + phosphorylated c-Jun | - |
? | |
| ATP + c-Jun | - |
Bos taurus | ADP + phosphorylated c-Jun | - |
? | |
| additional information | the enzyme is involved in both the inositol phosphate and the stress response pathways which are linked by the enzyme, overview | Bos taurus | ? | - |
? | |
| additional information | the enzyme is part of the COP9 signalosome complex consisting of 8 proteins | Homo sapiens | ? | - |
? | |
| additional information | the enzyme is part of the COP9 signalosome complex consisting of 8 proteins | Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
| 7.5 | - |
assay at | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
| ATP | - |
Bos taurus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00003 | - |
1D-myo-inositol-3,4,5,6-tetrakisphosphate | pH 7.5, 37°C | Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
