Any feedback?
Literature summary for 2.7.1.159 extracted from
- Isolation of inositol 1,3,4-trisphosphate 5/6-kinase, cDNA cloning, and expression of the recombinant enzyme (1996), J. Biol. Chem., 271, 11904-11910.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and N-terminal amino acid sequence determination and analysis | Bos taurus |
| DNA and N-terminal amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00008 | - |
1D-myo-inositol-1,3,4-trisphosphate | - |
Bos taurus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bos taurus | |
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45600 | - |
x * 45600, about, sequence calculation | Homo sapiens |
| 48000 | - |
x * 36000-38000, proteolytically C-terminally truncated enzyme, SDS-PAGE, x * 48000, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Homo sapiens | Q13572 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 12900fold from brain by ion exchange chromatography, and heparin/inositol hexakisphosphate affinity chromatography | Bos taurus |
| recombinant His-tagged enzyme from Escherichia coli by adsorption and calmodulin affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
| brain | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25 | 48 | calf brain | Bos taurus |
| 372 | - |
purified enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation | Bos taurus | ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | lower activity compared to 1D-myo-inositol-1,3,4,6-tetrakisphosphate formation | Homo sapiens | ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation | Bos taurus | ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol-1,3,4-trisphosphate | higher activity compared to 1D-myo-inositol-1,3,4,5-tetrakisphosphate formation | Homo sapiens | ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000-38000, proteolytically C-terminally truncated enzyme, SDS-PAGE, x * 48000, SDS-PAGE | Bos taurus |
| ? | x * 45600, about, sequence calculation | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bos taurus |
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bos taurus | |
| ATP | - |
Homo sapiens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | sequence calculation | - |
6 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
