Protein Variants | Comment | Organism |
---|---|---|
E82C/S142C | the mutant exhibits a dithiothreitol-sensitive 5fold increase in kcat for 3,4,5,6-inositol tetrakisphosphate compared to the wild type enzyme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04453 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme E82C/S142C, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.04754 | - |
1D-myo-inositol 3,4,5,6-tetrakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.06305 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.07917 | - |
1D-myo-inositol 3,4,5,6-tetrakisphosphate | mutant enzyme E82C/S142C, at pH 7.5 and 25°C | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | Arabidopsis thaliana | - |
ADP + 1D-myo-inositol hexakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
Arabidopsis thaliana | ADP + 1D-myo-inositol hexakisphosphate | - |
? | |
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate | low activity | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inositol 1,3,4,5,6-pentakisphosphate 2-kinase | - |
Arabidopsis thaliana |
Ipk1 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.105 | - |
1D-myo-inositol 3,4,5,6-tetrakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.549 | - |
1D-myo-inositol 3,4,5,6-tetrakisphosphate | mutant enzyme E82C/S142C, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.734 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.736 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme E82C/S142C, at pH 7.5 and 25°C | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
1D-myo-inositol 3,4,5,6-tetrakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
6.8 | - |
1D-myo-inositol 3,4,5,6-tetrakisphosphate | mutant enzyme E82C/S142C, at pH 7.5 and 25°C | Arabidopsis thaliana | |
11.7 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
16.5 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme E82C/S142C, at pH 7.5 and 25°C | Arabidopsis thaliana |