Cloned (Comment) | Organism |
---|---|
Cloning from a potato tuber cDNA library and expression of StITPK1-His protein in Escherichia coli rosetta cells. | Solanum tuberosum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | included in assay medium | Solanum tuberosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | Solanum tuberosum | the predominant activity of StITPK1 is that of an InsP5-ADP phosphotransferase. | ADP + 1D-myo-inositol hexakisphosphate | - |
? | |
additional information | Solanum tuberosum | StITPK1 displays inositol 3,4,5,6-tetrakisphosphate 1-kinase activity. The enzyme displays inositol 1,3,4,5,6-pentakisphosphate 1-phosphatase activity in the absence of a nucleotide acceptor and inositol 1,3,4,5,6-pentakisphosphate-ADP phosphotransferase activity in presence of physiological concentrations of ADP. Additionally, StITPK1 shows inositol phosphate-inositol phosphate phosphotransferase activity. | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | A4H2J0 | - |
- |
Purification (Comment) | Organism |
---|---|
by batch-elution from Ni-NTA affinity resin, Analysis by SDS-PAGE gel and Western Blot using an anti-His antibody. Purity of protein is >98% estimated by SDS-PAGE. | Solanum tuberosum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | expression of Solanum tuberosum protein in Escherichia coli | Solanum tuberosum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | the predominant activity of StITPK1 is that of an InsP5-ADP phosphotransferase. | Solanum tuberosum | ADP + 1D-myo-inositol hexakisphosphate | - |
? | |
additional information | StITPK1 displays inositol 3,4,5,6-tetrakisphosphate 1-kinase activity. The enzyme displays inositol 1,3,4,5,6-pentakisphosphate 1-phosphatase activity in the absence of a nucleotide acceptor and inositol 1,3,4,5,6-pentakisphosphate-ADP phosphotransferase activity in presence of physiological concentrations of ADP. Additionally, StITPK1 shows inositol phosphate-inositol phosphate phosphotransferase activity. | Solanum tuberosum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inositol polyphosphate kinase-1 | - |
Solanum tuberosum |
inositol polyphosphate kinase/phosphotransferase | multifunctional | Solanum tuberosum |
StITPK1 | - |
Solanum tuberosum |
StITPKalpha | - |
Solanum tuberosum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Solanum tuberosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Solanum tuberosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Solanum tuberosum |