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Literature summary for 2.7.1.158 extracted from

  • Sun, Y.; Thompson, M.; Lin, G.; Butler, H.; Gao, Z.; Thornburgh, S.; Yau, K.; Smith, D.A.; Shukla, V.K.
    Inositol 1,3,4,5,6-pentakisphosphate 2-kinase from maize: molecular and biochemical characterization (2007), Plant Physiol., 144, 1278-1291.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloning and expression in Escherichia coli Zea mays
expression in Escherichia coli Zea mays

General Stability

General Stability Organism
accumulation of soluble ZmIPK1 protein is very low under various growing temperatures and induction conditions, and the majority of the fusion protein is detected in inclusion bodies. Therefore, an alternative strategy using chaperone protein coexpression to enhance ZmIPK1 protein solubility is exploited. Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.119
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
Zea mays
0.119
-
myo-inositol 1,3,4,5,6-pentakisphosphate
-
Zea mays
0.119
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate 400 microM of substrate ATP is used Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
inclusion body The majority of fusion protein is detected. Zea mays 16234
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48850
-
predicted Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP Zea mays intermediate in both lipid-dependent and lipid-independent phytic acid biosynthetic pathways 1D-myo-inositol hexakisphosphate + ADP phytic acid, in higher plants, phytic acid can mediate abscisic acid-induced guard cell closure by inactivating plasma membrane inward K+ conductance. During this process, phytic acid appears to act as an endomembrane calcium-releasing signal. ir
1D-myo-inositol 1,4,6-trisphosphate + ATP Zea mays relative substrate specificity 93.9% 1D-myo-inositol 1,2,4,6-tetrakisphosphate + ADP
-
?
additional information Zea mays capable to catalyze the phosphorylation of myo-inositol 1,4,5,6-tetrakisphosphate (relative substrate specificity 76.8%), and myo-inositol 3,4,5,6-tetrakisphosphate (relative substrate specificity 32.6%) ?
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-
Zea mays A4GWX8
-
-
Zea mays A4GWX8 Alignments of the leaf- and seed-derived cDNA sequences with ZmIPK1A define nine predicted exons and eight predicted introns in theZmIPK1 genomic sequence
-
Zea mays A6YH13
-
-
Zea mays A6YH13 contains a 31 bp deletion and a 7 bp polymorphism when compared to ZmIPK1A
-

Purification (Commentary)

Purification (Comment) Organism
a glutathione affinity column is used to capture intact GST-ZmIPK1. The purified GST-ZmIPK1 fusion is cleaved with thrombin and further purified by a sequential affinity and ion-exchange chromatography. ZmIPK1 protein is eluted at 0.1 M NaCl on a high resolution Mono Q column with an estimated purity of 95%. Identity of the purified protein is confirmed via trypsin digestion followed by matrix-assisted laser deposition/ionization (MALDI) time-of-flight mass spectrometry analysis and comparison of the MALDI fingerprint to the amino acid sequences deduced from ZmIPK1. Zea mays
expression in Escherichia coli Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
cob immature ear Zea mays
-
corn silk
-
Zea mays
-
embryo mature Zea mays
-
embryo maturing, ZmIPK1A Zea mays
-
endosperm midstage, ZmIPK1A Zea mays
-
endosperm midstage Zea mays
-
leaf
-
Zea mays
-
leaf a variety of alternative splicing products of ZmIPK1A are discovered in maize leaves and seeds Zea mays
-
leaf A variety of alternative splicing products of ZmIPK1A are discovered in maize leaves and seeds. The products are derived from alternative acceptor sites, alternative donor sites, and retained introns in the transcripts. Up to 50% of the ZmIPK1A transcripts in maize seeds and leaves have an interrupted open reading frame. One type of splicing product of ZmIPK1B is detected in roots. Zea mays
-
additional information A variety of alternative splicing products of ZmIPK1A are discovered in maize leaves and seeds. Products are derived from alternative acceptor sites, alternative donor sites, and retained introns in the transcripts. Up to 50% of the ZmIPK1A transcripts in maize seeds and leaves have an interrupted open reading frame. Zea mays
-
root
-
Zea mays
-
root transcripts of ZmIPK1B were exclusively detected in roots Zea mays
-
root transcripts detected in ZmIPK1B Zea mays
-
seed
-
Zea mays
-
seed a variety of alternative splicing products of ZmIPK1A are discovered in maize leaves and seeds, ZmIPK1A Zea mays
-
seed at 12 d after pollination Zea mays
-
silk ZmIPK1A Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.625
-
-
Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP intermediate in both lipid-dependent and lipid-independent phytic acid biosynthetic pathways Zea mays 1D-myo-inositol hexakisphosphate + ADP phytic acid, in higher plants, phytic acid can mediate abscisic acid-induced guard cell closure by inactivating plasma membrane inward K+ conductance. During this process, phytic acid appears to act as an endomembrane calcium-releasing signal. ir
1D-myo-inositol 1,4,6-trisphosphate + ATP relative substrate specificity 93.9% Zea mays 1D-myo-inositol 1,2,4,6-tetrakisphosphate + ADP
-
?
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
Zea mays ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
-
Zea mays ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
?
ATP + myo-inositol 1,3,4,5,6-pentakisphosphate
-
Zea mays ADP + myo-inositol hexakisphosphate
-
?
ATP + myo-inositol 1,4,6-trisphosphate
-
Zea mays ADP + inositol 1,2,4,6-tetrakisphosphate
-
?
additional information capable to catalyze the phosphorylation of myo-inositol 1,4,5,6-tetrakisphosphate (relative substrate specificity 76.8%), and myo-inositol 3,4,5,6-tetrakisphosphate (relative substrate specificity 32.6%) Zea mays ?
-
?

Synonyms

Synonyms Comment Organism
inositol 1,3,4,5,6-pentakisphosphate 2-kinase
-
Zea mays
Ipk1
-
Zea mays
ZmIPK1
-
Zea mays
ZmIPK1A
-
Zea mays
ZmIPK1A nearly identical paralog Zea mays
ZmIPK1A nearly identical paralog to ZmIPK1, genomic ZmIPK1A sequence contains only one EcoRI site Zea mays
ZmIPK1B
-
Zea mays
ZmIPK1B nearly identical paralog Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Zea mays

Cofactor

Cofactor Comment Organism Structure
ATP
-
Zea mays

pI Value

Organism Comment pI Value Maximum pI Value
Zea mays predicted
-
7.8