Cloned (Comment) | Organism |
---|---|
real-time PCR analysis of PI3K-C2alpha and PI3K-C2beta in cells transfected with the control or shRNA expression vector NPY-mRFP and EGFP-PI3K-C2alpha are transfected into RBL-2H3 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
yes | knockout of PI3K-C2alpha and PI3K-C2beta by shRNA expression | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Wortmannin | a pan-PI3K inhibitor | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vesicle | existence of PI3K-C2alpha and 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate on CD63-positive large vesicles in FceRI-stimulated RBL-2H3 cells | Rattus norvegicus | 31982 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
RBL-2H3 cell | - |
Rattus norvegicus | - |
Synonyms | Comment | Organism |
---|---|---|
class II phosphoinositide 3-kinase alpha | - |
Rattus norvegicus |
PI3K-C2alpha | - |
Rattus norvegicus |
PI3K-C2beta | - |
Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00042 | - |
isozyme PI3K-C2alpha, pH and temperature not specified in the publication | Rattus norvegicus | Wortmannin |
General Information | Comment | Organism |
---|---|---|
malfunction | PI3K-C2alpha knockdown decreases the FceRI-triggered release of a lysosomal enzyme, namely beta-hexosaminidase, protein levels of PI3K-C2beta are not significantly affected by the shRNAs. siRNA against PI3K-C2beta impairs the FceRI-induced increase in intracellular Ca2+ and degranulation. Release of NPY-mRFP, a reporter of the regulated exocytosis of mast cells is increased by PI3K-C2alpha overexpression and is impaired in the PI3K-C2alpha-deficient cells | Rattus norvegicus |
metabolism | involvement of PI3K-C2alpha in the antigen-induced degranulation pathway | Rattus norvegicus |
physiological function | PI3K-C2beta regulates the FceRI-stimulated activation of the KCa3.1 channel and the FceRI-induced Ca2+ influx and degranulation in BMMCs. PI3K-C2alpha regulates the FceRI-triggered degranulation by a mechanism different from that induced by PI3K-C2beta. PI3KC2alpha and PI3K-C2beta share different roles in the FceRI-regulated degranulation pathway | Rattus norvegicus |