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Literature summary for 2.7.1.154 extracted from

  • Molz, L.; Chen, Y.W.; Hirano, M.; Williams, L.T.
    CpK is a novel class of Drosophila PtdIns 3-kinase containing a C2 domain (1996), J. Biol. Chem., 271, 13892-13899.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-7 cells Mus musculus
expression in COS-7 cells. Specific activity of the enzyme purified from Drosophila lysate is significantly higher than that of Cpk derived from exogenous expression in COS-7 cells Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
Wortmannin IC50: 11 nM Drosophila melanogaster

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme strictly requires the presence of Mg2+, inactive in presence of Mn2+ Drosophila melanogaster
additional information inactive in presence of Mn2+ Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q7JNX6
-
-
Mus musculus Q61194
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol-4-phosphate
-
Drosophila melanogaster ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
-
?
ATP + phosphatidylinositol
-
Drosophila melanogaster ADP + phosphatidylinositol 3-phosphate
-
?
additional information no activity with phosphatidylinositol 4,5-diphosphate Drosophila melanogaster ?
-
?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000011
-
IC50: 11 nM Drosophila melanogaster Wortmannin