Literature summary for 2.7.1.154 extracted from

Arcaro, A.; Volinia, S.; Zvelebil, M.J.; Stein, R.; Watton, S.J.; Layton, M.J.; Gout, I.; Ahmadi, K.; Downward, J.; Waterfield, M.D.
Human phosphoinositide 3-kinase C2b, the role of calcium and the C2 domain in enzyme activity (1998), J. Biol. Chem., 273, 33082-33090.

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Cloned(Commentary)

Cloned (Comment)	Organism
Glu-tagged PI 3-kinase C2beta protein is expressed in both HEK293 cells and Sf9 cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Wortmannin	IC50: 1.6 nM	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.12	-	ATP	pH 7.4	Homo sapiens
0.12	-	ATP	ATP in form of CaATP2-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	the recombinant enzyme is localized mainly in the low density microsomal fraction, with a small amount being present in the plasma membrane and the cytosolic fraction	Homo sapiens	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	phosphorylation of phosphatidylinositol but not phosphatidylinositol 4-phosphate in presence of Ca2+	Homo sapiens
Mg2+	phosphorylation of phosphatidylinositol and phosphatidylinositol 4-phosphate in presence of Mg2+	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
182000	-	x * 182000, calculation from nucleotide sequence	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O00750	PI3K-C2beta	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
U-937 cell	monocyte	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate	-	Homo sapiens	ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate	-	?
ATP + 1-phosphatidyl-1D-myo-inositol-4-phosphate	at 10% of the activity with phosphatidylinositol, phosphorylation only in presence of Mg2+, not in presence of Ca2+	Homo sapiens	ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate	-	?
ATP + phosphatidylinositol	-	Homo sapiens	ADP + phosphatidylinositol 3-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 182000, calculation from nucleotide sequence	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0000016	-	IC50: 1.6 nM	Homo sapiens	Wortmannin

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Release 2023.1 (January 2023)