Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | virulent Legionella pneumophila infection activates PI3K 10fold in vivo, the avirulent bacteria strain Lp-14 activates 4fold in vivo | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
overexpression of the dominant negative PI3K mutant in J774A.1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a dominant negative PI3K mutant by PCR cloning. Legionella pneumophila entry into macrophages expressing the mutant is reduced | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
LY294002 | - |
Homo sapiens | |
Wortmannin | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | Homo sapiens | - |
ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
macrophage | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | p85alpha subunit of class I PI3K | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PI3K | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000025 | - |
inhibition of bacterial entry into macrophages | Homo sapiens | Wortmannin | |
0.008 | - |
inhibition of bacterial entry into macrophages | Homo sapiens | LY294002 |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | virulent Legionella pneumophila infection induces PI3K in human macrophages, while PI3K and protein kinase B, PKB/Akt, activities are lower in macrophages infected with an avirulent bacterial strain | up |
General Information | Comment | Organism |
---|---|---|
physiological function | phosphatidylinositol 3-kinase mediates non-opsonic phagocytosis of Legionella pneumophila by macrophages | Homo sapiens |