Literature summary for 2.7.1.151 extracted from

Zhang, Z.B.; Yang, G.; Arana, F.; Chen, Z.; Li, Y.; Xia, H.J.
Arabidopsis inositol polyphosphate 6-/3-kinase (AtIpk2beta) is involved in axillary shoot branching via auxin signaling (2007), Plant Physiol., 144, 942-951.

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Activating Compound

Activating Compound	Comment	Organism	Structure
indole-3-acetic acid	The expression of AtIpk2b is enhanced in roots after treatment with 1 microM. Expression of AtIpk2b become stronger when the concentration of exogenous indole-3-acetic acid is increased to 10 microM. By treatment with 40 microM, AtIpk2b expression increases after 0.5 h of treatment, whereas the expression of AtIpk2a remains virtually unaffected. These results indicate that AtIpk2b is an IAA-inducible gene, while AtIpk2a is not.	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
To create an AtIpk2b overexpression construct, the AtIpk2b cDNA is cut from plasmid pmIP3K with XbaI-XhoI and inserted via XbaI-SalI sites into pBinAR-HPT vector behind the cauliflower mosaic virus 35S promoter. The resulting plasmid is first introduced into Agrobacterium tumefaciens by electroporation and then transformed Arabidopsis ecotype C24 by vacuum infiltration. Transgenic plants are selected on MS medium containing 20 mg/L hygromycin. Eleven AtIpk2b overexpression plants, named OX-2, OX-3, OX-5, OX-8, OX-9, OX-15, OX-25, OX-26, OX-33, OX-35, and OX-64, are identified. Compared to the wild type, branching of axillary shoots is more pronounced in the overexpression lines.	Arabidopsis thaliana

Cloned (Comment)

Organism

To create an AtIpk2b overexpression construct, the AtIpk2b cDNA is cut from plasmid pmIP3K with XbaI-XhoI and inserted via XbaI-SalI sites into pBinAR-HPT vector behind the cauliflower mosaic virus 35S promoter. The resulting plasmid is first introduced into Agrobacterium tumefaciens by electroporation and then transformed Arabidopsis ecotype C24 by vacuum infiltration. Transgenic plants are selected on MS medium containing 20 mg/L hygromycin. Eleven AtIpk2b overexpression plants, named OX-2, OX-3, OX-5, OX-8, OX-9, OX-15, OX-25, OX-26, OX-33, OX-35, and OX-64, are identified. Compared to the wild type, branching of axillary shoots is more pronounced in the overexpression lines.

Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Arabidopsis thaliana	AtIpk2b Is an auxin-inducible gene and is important for the auxin signaling pathway	?	-	?
additional information	Arabidopsis thaliana	IP3K is involved in axillary shoot branching via Auxin signaling	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9LY23	ecotypes C24 and Columbia	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-
root hair	-	Arabidopsis thaliana	-
root tip	-	Arabidopsis thaliana	-
silique	-	Arabidopsis thaliana	-
stem	-	Arabidopsis thaliana	-
stigma	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	AtIpk2b Is an auxin-inducible gene and is important for the auxin signaling pathway	Arabidopsis thaliana	?	-	?
additional information	IP3K is involved in axillary shoot branching via Auxin signaling	Arabidopsis thaliana	?	-	?

Synonyms

Synonyms	Comment	Organism
AtIpk2a	-	Arabidopsis thaliana
AtIpk2a	gene of isoform alpha, 84% similarity and 73% identity with AtIpk2b	Arabidopsis thaliana
AtIpk2b	-	Arabidopsis thaliana
AtIpk2b	gene of isoform beta, 84% similarity and 73% identity with AtIpk2a	Arabidopsis thaliana
inositol 1,4,5-trisphosphate 3-kinase	-	Arabidopsis thaliana
inositol polyphosphate 6-/3-kinase	-	Arabidopsis thaliana
IP3K	-	Arabidopsis thaliana