Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, overexpression as C-terminally GFP-tagged fusion protein in Escherichia coli strain BL21(DE3), transient expression in NRK 52E cells as C- or N-terminally GFP-tagged fusion protein | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00028 | - |
1D-myo-inositol 1,4,5-trisphosphate | pH 7.8, 30°C, 0.5 mM ATP, recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | predominantly, a basic cluster at the enzyme's C-terminus is involved in nuclear targeting | Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Homo sapiens | |
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47219 | - |
x * 47219, amino acid sequence calculation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8NFU5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by ion exchange and phosphocellulose resin chromatography | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | the catalytic site contains an important Ser-Ser-Leu-Leu motif | Homo sapiens | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | the catalytic site contains an important Ser-Ser-Leu-Leu motif | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.45 | - |
purified recombinant enzyme, 1D-myo-inositol 1,3,4,5,6-pentakisphosphate formation from 1D-myo-inositol 1,4,5-trisphosphate, 0.5 mM ATP | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Homo sapiens | 2 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Homo sapiens | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
ir | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Homo sapiens | ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 47219, amino acid sequence calculation | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
inositol phosphate multikinase | - |
Homo sapiens |
IPMK | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |