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Literature summary for 2.7.1.151 extracted from

  • Chang, S.C.; Miller, A.L.; Feng, Y.; Wente, S.R.; Majerus, P.W.
    The human homolog of the rat inositol phosphate multikinase is an inositol 1,3,4,6-tetrakisphosphate 5-kinase (2002), J. Biol. Chem., 277, 43836-43843.
    View publication on PubMed
Search for more literature for 2.7.1.151

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, no functional complementation of yeast mutant strains, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Homo sapiens
0.00011
-
 1D-myo-inositol 1,4,5-trisphosphate recombinant enzyme, pH 7.2, 37°C Homo sapiens
0.00013
-
 1D-myo-inositol 1,3,4,5-tetrakisphosphate recombinant enzyme, pH 7.2, 37°C Homo sapiens
0.0003
-
 1D-myo-inositol 1,3,4,6-tetrakisphosphate recombinant enzyme, pH 7.2, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate Homo sapiens
-
 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate Homo sapiens
-
 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate 43fold less active compared to 1D-myo-inositol 1,3,4,6-tetrakisphosphate Homo sapiens ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate preferred substrate Homo sapiens ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate 2fold less active compared to 1D-myo-inositol 1,3,4,6-tetrakisphosphate Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate 2fold less active compared to 1D-myo-inositol 1,3,4,6-tetrakisphosphate Homo sapiens ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?
additional information substrate specificity, product identification Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
1,3,4,6-tetrakisphosphate 5-kinase
-
 Homo sapiens
inositol phosphate multikinase
-
 Homo sapiens
InsP4 5-kinase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP enzyme contains the putative binding site P-X-X-X-D-X-K-X-G Homo sapiens