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Literature summary for 2.7.1.15 extracted from

  • Quiroga-Roger, D.; Babul, J.; Guixe, V.
    Role of monovalent and divalent metal cations in human ribokinase catalysis and regulation (2015), Biometals, 28, 401-413.
    View publication on PubMed
Search for more literature for 2.7.1.15

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E202L mutation in NXXE motif, increase in the Km for MgATP of 52fold, and a decrease in the kcat value of around 200fold Homo sapiens
N199L mutation in NXXE motif, increase in the Km for MgATP- by about 68fold, a decrease in the kcat between 573- and 1036fold and almost no change in the Km for D-ribose. No substrate inhibition by D-ribose Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
 Homo sapiens
D-ribose substrate inhibition Homo sapiens
EDTA complete inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 ATP wild-type, presence of Mn2+, pH 7.2, 37°C Homo sapiens
0.08
-
 D-ribose mutant E202L, presence of Mg2+, pH 7.2, 37°C Homo sapiens
0.12
-
 D-ribose mutant N199L, presence of Mg2+, pH 7.2, 37°C Homo sapiens
0.12
-
 D-ribose wild-type, presence of Mg2+, pH 7.2, 37°C Homo sapiens
0.16
-
 ATP wild-type, presence of Mg2+, pH 7.2, 37°C Homo sapiens
5.73
-
 ATP mutant E202L, presence of Mg2+, pH 7.2, 37°C Homo sapiens
7.48
-
 ATP mutant N199L, presence of Mg2+, pH 7.2, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ may partly support activity Homo sapiens
Co2+ about 60% of the activity with Mn2+ Homo sapiens
Mg2+ about 70% of the activity with Mn2+ Homo sapiens
Mn2+ best activator, optimum concentration 0.1 mM Homo sapiens
Ni2+ may partly support activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H477
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose
-
 Homo sapiens ADP + D-ribose 5-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 ATP mutant N199L, presence of Mg2+, pH 7.2, 37°C Homo sapiens
0.01
-
 D-ribose mutant N199L, presence of Mg2+, pH 7.2, 37°C Homo sapiens
0.04
-
 ATP mutant E202L, presence of Mg2+, pH 7.2, 37°C Homo sapiens
0.04
-
 D-ribose mutant E202L, presence of Mg2+, pH 7.2, 37°C Homo sapiens
7.3
-
 ATP wild-type, presence of Mn2+, pH 7.2, 37°C Homo sapiens
9.8
-
 ATP wild-type, presence of Mg2+, pH 7.2, 37°C Homo sapiens
10.4
-
 D-ribose wild-type, presence of Mg2+, pH 7.2, 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
10.3
-
 ATP wild-type, presence of Mn2+, pH 7.2, 37°C Homo sapiens
16.9
-
 ATP wild-type, presence of Mg2+, pH 7.2, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0013
-
 ATP mutant N199L, presence of Mg2+, pH 7.2, 37°C Homo sapiens
0.007
-
 ATP mutant E202L, presence of Mg2+, pH 7.2, 37°C Homo sapiens
0.08
-
 D-ribose mutant N199L, presence of Mg2+, pH 7.2, 37°C Homo sapiens
0.5
-
 D-ribose mutant E202L, presence of Mg2+, pH 7.2, 37°C Homo sapiens
60
-
 ATP wild-type, presence of Mg2+, pH 7.2, 37°C Homo sapiens
90
-
 D-ribose wild-type, presence of Mg2+, pH 7.2, 37°C Homo sapiens
250
-
 ATP wild-type, presence of Mn2+, pH 7.2, 37°C Homo sapiens