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Literature summary for 2.7.1.15 extracted from

  • Agranoff, B.W.; Brady, R.O.
    Purification and properties of calf liver ribokinase (1956), J. Biol. Chem., 219, 221-229.
    View publication on PubMed
Search for more literature for 2.7.1.15

Inhibitors

Inhibitors Comment Organism Structure
Na+ 0.02 M, 30% inhibition Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 D-ribose 30°C, pH 7 Bos taurus
0.035
-
 2-deoxy-D-ribose 30°C, pH 7 Bos taurus
0.04
-
 ATP 30°C, pH 7, in presence of Mg2+ Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Bos taurus
Mg2+ absolute requirement for divalent cations, best satisfied by Mg2+ Bos taurus
Mn2+ can replace Mg2+ in activation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.2
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-12°C fraction IV retains 65% activity after two months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-ribose
-
 Bos taurus ADP + 2-deoxy-D-ribose 5-phosphate
-
 ?
ATP + D-ribose
-
 Bos taurus ADP + D-ribose 5-phosphate
-
 ?
CTP + D-ribose
-
 Bos taurus CDP + D-ribose 5-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1 7.8
-
 Bos taurus