Application | Comment | Organism |
---|---|---|
drug development | enzymes of the MEP pathway represent potential targets for the generation of selective antibacterial, antimalarial and herbicidal molecules | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
the structure is resolved by X-ray diffraction at a resolution of 2.01 A | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
determined by MALDI-TOF-MS | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | Escherichia coli | - |
ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Hi-trap chelating columns are used for the purification of recombinant 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
Escherichia coli | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | determined by gel filtration, asymmetric unit consists of 2 subunits A and B which assemble with C2 symmetry to form an extended homodimer, but only 4% of the total surface area of the 2 monomers is involved in dimer formation | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase | - |
Escherichia coli |
CDP-methylerythritol kinase | - |
Escherichia coli |
CMK | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme catalyses the fourth reaction step of the 2C-methylerythritol 4-phosphate (MEP) pathway for the biosynthesis of isopentenyl pyrophosphate and its isomer dimethylallyl pyrophosphate | Escherichia coli |
physiological function | the MEP pathway is essential in the malaria parasite Plasmodium falciparum and in most eubacteria, including the causal agents for diverse and serious human diseases like leprosy, bacterial meningitis, various gastrointestinal and sexually transmitted infections, tuberculosis, and certain types of pneumonia | Escherichia coli |