Crystallization (Comment) | Organism |
---|---|
crystal structures of apo form and holo form, in the presence of D-glucose and the nonhydrolyzable ADP analog adenosine 5'-(3-thio)diphosphate. The conformational changes upon sequential substrate binding can be explained by an almost pure rotation (or a rotation plus a translation) facilitated by residues in the flexible inter-domain connection | Thermococcus litoralis |
sitting-drop vapor-diffusion crystallization | Thermococcus litoralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0086 | - |
ADP | pH not specified in the publication, temperature not specified in the publication | Thermococcus litoralis | |
0.0086 | - |
ADP | 40°C, pH 7.8 | Thermococcus litoralis | |
0.218 | - |
D-glucose | pH not specified in the publication, temperature not specified in the publication | Thermococcus litoralis | |
0.219 | - |
D-glucose | 40°C, pH 7.8 | Thermococcus litoralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | Q7M537 | - |
- |
Thermococcus litoralis DSM 5473 | Q7M537 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + D-glucose | - |
Thermococcus litoralis | AMP + D-glucose 6-phosphate | - |
? | |
ADP + D-glucose | catalysis follows a sequential ordered mechanism | Thermococcus litoralis | AMP + D-glucose 6-phosphate | - |
? | |
ADP + D-glucose | - |
Thermococcus litoralis DSM 5473 | AMP + D-glucose 6-phosphate | - |
? | |
ADP + D-glucose | catalysis follows a sequential ordered mechanism | Thermococcus litoralis DSM 5473 | AMP + D-glucose 6-phosphate | - |
? | |
additional information | catalysis follows a sequential ordered mechanism | Thermococcus litoralis | ? | - |
? | |
additional information | catalysis follows a sequential ordered mechanism | Thermococcus litoralis DSM 5473 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADP-dependent glucokinase | - |
Thermococcus litoralis |