Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.147 extracted from

  • Koga, S.; Yoshioka, I.; Sakuraba, H.; Takahashi, M.; Sakasegawa, S.; Shimizu, S.; Ohshima, T.
    Biochemical characterization, cloning, and sequencing of ADP-dependent (AMP-forming) glucokinase from two hyperthermophilic archaea, Pyrococcus furiosus and Thermococcus litoralis (2000), J. Biochem., 128, 1079-1085.
    View publication on PubMed
Search for more literature for 2.7.1.147

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 93% of activity Pyrococcus furiosus
Co2+ 22% of activity Thermococcus litoralis
Mg2+ required for activation Pyrococcus furiosus
Mg2+ required for activation Thermococcus litoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000 Pyrococcus furiosus
52000
-
 1 * 52000 Thermococcus litoralis
54000
-
 gel filtration Thermococcus litoralis
100000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + ADP Pyrococcus furiosus CDP shows comparable activity D-glucose 6-phosphate + AMP
-
 ir
D-glucose + ADP Thermococcus litoralis CDP shows comparable activity D-glucose 6-phosphate + AMP
-
 ir

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-
Thermococcus litoralis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
166
-
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-1,5-anhydroglucitol + ADP
-
 Pyrococcus furiosus ?
-
 ?
D-1,5-anhydroglucitol + ADP
-
 Thermococcus litoralis ?
-
 ?
D-glucose + ADP CDP shows comparable activity Pyrococcus furiosus D-glucose 6-phosphate + AMP
-
 ir
D-glucose + ADP CDP shows comparable activity Thermococcus litoralis D-glucose 6-phosphate + AMP
-
 ir

Subunits

Subunits Comment Organism
dimer 2 * 47000 Pyrococcus furiosus
monomer 1 * 52000 Thermococcus litoralis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
-
 Thermococcus litoralis
90
-
 at least stable for 10 min Pyrococcus furiosus