Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.146 extracted from

  • Merino, F.; Rivas-Pardo, J.A.; Caniuguir, A.; Garcia, I.; Guixe, V.
    Catalytic and regulatory roles of divalent metal cations on the phosphoryl-transfer mechanism of ADP-dependent sugar kinases from hyperthermophilic archaea (2012), Biochimie, 94, 516-524.
    View publication on PubMed
Search for more literature for 2.7.1.146

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 ADP presence of Mg2+, pH 6.5, 50°C Pyrococcus horikoshii
0.008
-
 ADP presence of Mn2+, pH 6.5, 50°C Pyrococcus horikoshii
0.022
-
 ADP presence of Co2+, pH 6.5, 50°C Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
additional information divalent cation is strictly required for activity, the true substrate seems to be the metal-nucleotide complex. The enzyme is promiscuous in relation to its metal usage where the only considerations for metal assisted catalysis seem to be related to the ionic radii and coordination geometry of the cations. The metal cation is bound to the highly conserved NXXE motif, which constitutes one of the signatures of the ribokinase superfamily. The binding of a second metal to the enzyme produces a complex with a reduced catalytic constant Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + D-fructose 6-phosphate
-
 Pyrococcus horikoshii AMP + D-fructose 1,6-bisphosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
52
-
 ADP presence of Mg2+, pH 6.5, 50°C Pyrococcus horikoshii
67
-
 ADP presence of Mn2+, pH 6.5, 50°C Pyrococcus horikoshii
98
-
 ADP presence of Co2+, pH 6.5, 50°C Pyrococcus horikoshii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4400
-
 ADP presence of Co2+, pH 6.5, 50°C Pyrococcus horikoshii
6500
-
 ADP presence of Mg2+, pH 6.5, 50°C Pyrococcus horikoshii
8400
-
 ADP presence of Mn2+, pH 6.5, 50°C Pyrococcus horikoshii