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Literature summary for 2.7.1.146 extracted from
- ADP-dependent 6-phosphofructokinase, an extremely thermophilic, non-allosteric enzyme from the hyperthermophilic, sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324 (2004), Extremophiles, 8, 29-35.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no allosterical regulation by ADP, AMP, phosphoenolpyruvate, or citrate | Archaeoglobus fulgidus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no allosterical regulation by ADP, AMP, phosphoenolpyruvate, or citrate | Archaeoglobus fulgidus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
ADP | pH 6.6, 50°C | Archaeoglobus fulgidus |  |
| 0.6 | - |
ADP | pH 6.6, 80°C | Archaeoglobus fulgidus | |
| 1.4 | - |
AMP | pH 6.6, 80°C | Archaeoglobus fulgidus | |
| 2.2 | - |
D-fructose 6-phosphate | pH 6.6, 80°C | Archaeoglobus fulgidus | |
| 4.7 | - |
D-fructose 6-phosphate | pH 6.6, 50°C | Archaeoglobus fulgidus | |
| 5.7 | - |
D-fructose 1,6-bisphosphate | pH 6.6, 80°C | Archaeoglobus fulgidus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Archaeoglobus fulgidus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 30% of activity with Mg2+ | Archaeoglobus fulgidus | |
| Fe2+ | 16% of activity with Mg2+ | Archaeoglobus fulgidus | |
| Mg2+ | most efficient divalent cation, can be replaced by Ni2+, Co2+, and Mn2+ | Archaeoglobus fulgidus | |
| Mn2+ | 62% of activity with Mg2+ | Archaeoglobus fulgidus | |
| additional information | divalent cations are required for activity, Zn2+, Ca2+, and Cu2+ are poor metal cofactors | Archaeoglobus fulgidus | |
| Ni2+ | 33% of activity with Mg2+ | Archaeoglobus fulgidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Archaeoglobus fulgidus |
| 50000 | - |
4 * 50000, SDS-PAGE | Archaeoglobus fulgidus |
| 100000 | - |
dimeric enzyme form, gel filtration at higher protein concentration | Archaeoglobus fulgidus |
| 200000 | - |
tetrameric enzyme form, gel filtration at lower protein concentration | Archaeoglobus fulgidus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + D-fructose 6-phosphate | Archaeoglobus fulgidus | - |
AMP + D-fructose 1,6-bisphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
strain 7324, no activity in strain VC 16 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1818fold to homogeneity by ultracentrifugation, anion exchange, ADP affinity, and cation exchange chromatography, and gel filtration | Archaeoglobus fulgidus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12 | - |
purified enzyme, determined at pH 6.6, 80°C, reverse reaction | Archaeoglobus fulgidus |
| 392 | - |
purified enzyme, determined at pH 6.6, 50°C, forward reaction | Archaeoglobus fulgidus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified native enzyme, quite stable | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + D-fructose 6-phosphate | - |
Archaeoglobus fulgidus | AMP + D-fructose 1,6-bisphosphate | - |
r | |
| ADP + D-fructose 6-phosphate | enzyme is highly specific for D-fructose 6-phosphate in the forward reaction | Archaeoglobus fulgidus | AMP + D-fructose 1,6-bisphosphate | - |
r | |
| additional information | no activity with ATP, diphosphate, or acetyl phosphate as phosphate donors, no activity with D-glucose as phosphate acceptor substrate | Archaeoglobus fulgidus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Archaeoglobus fulgidus |
| tetramer | 4 * 50000, SDS-PAGE | Archaeoglobus fulgidus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADP-dependent 6-phosphofructokinase | - |
Archaeoglobus fulgidus |
| ADP-Pfk | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Archaeoglobus fulgidus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 100 | - |
Archaeoglobus fulgidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
120 min, completely stable | Archaeoglobus fulgidus |
| 100 | - |
120 min, completely loss of activity after 60 min, in presence of 1 M NaCl the enzyme retains 30% activity after 90 min, in presence of 1 M KCl the enzyme retains 50% activity after 90 min | Archaeoglobus fulgidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Archaeoglobus fulgidus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.9 | 9 | 50% of maximal activity at pH 6.1 and pH 7.9 | Archaeoglobus fulgidus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Archaeoglobus fulgidus | |
| additional information | no activity with ATP, diphosphate, or acetyl phosphate | Archaeoglobus fulgidus |
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Release 2023.1 (January 2023)
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