Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 10 mM, 76% inhibition | Methanocaldococcus jannaschii | |
ATP | 10 mM, 47% inhibition | Methanocaldococcus jannaschii | |
KCl | 500 mM, 16% inhibition | Methanocaldococcus jannaschii | |
additional information | the addition of fructose 2,6-bisphosphate, pyruvate, glucose, phosphoenolpyruvate, or citrate to the assay mixture has no effect on the activity | Methanocaldococcus jannaschii | |
NaCl | 500 mM, 12% inhibition | Methanocaldococcus jannaschii | |
Zn2+ | no activity detected in presence of Zn2+ | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0096 | - |
D-fructose 6-phosphate | pH 6.5, 50°C | Methanocaldococcus jannaschii | |
0.49 | - |
ADP | pH 6.5, 50°C | Methanocaldococcus jannaschii | |
11.9 | - |
acetyl-phosphate | pH 6.5, 50°C | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Methanocaldococcus jannaschii | |
Ca2+ | activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ | Methanocaldococcus jannaschii | |
Co2+ | activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ | Methanocaldococcus jannaschii | |
Mg2+ | - |
Methanocaldococcus jannaschii | |
Mg2+ | activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ | Methanocaldococcus jannaschii | |
Mn2+ | activity is highest in the presence of CaCl2, followed by MgCl2, Co2+ and Mn2+ | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50100 | - |
native PAGE | Methanocaldococcus jannaschii |
50100 | - |
non-denaturing PAGE | Methanocaldococcus jannaschii |
53362 | - |
1 * 53362, calculated from sequence | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + D-fructose 6-phosphate | Methanocaldococcus jannaschii | key enzyme of the glycolytic pathway | AMP + D-fructose 1,6-bisphosphate | - |
? | |
ADP + D-fructose 6-phosphate | Methanocaldococcus jannaschii DSM 2661 | key enzyme of the glycolytic pathway | AMP + D-fructose 1,6-bisphosphate | - |
? | |
D-fructose 6-phosphate + ADP | Methanocaldococcus jannaschii | ADP can be replaced by acetylphosphate | D-fructose 1,6-bisphosphate + AMP | - |
? | |
D-fructose 6-phosphate + ADP | Methanocaldococcus jannaschii | ADP can be replaced by acetylphosphate | D-fructose 1,6-bisphosphate + AMP | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Methanocaldococcus jannaschii | Q58999 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58999 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Methanocaldococcus jannaschii |
8.2 | - |
pH 6.5, 50°C, direction of phosphorylation | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-phosphate + D-fructose 6-phosphate | activity is 83% compared to the activity with ADP | Methanocaldococcus jannaschii | acetate + D-fructose 1,6-bisphosphate | - |
? | |
acetyl-phosphate + D-fructose 6-phosphate | activity is 83% compared to the activity with ADP | Methanocaldococcus jannaschii DSM 2661 | acetate + D-fructose 1,6-bisphosphate | - |
? | |
ADP + D-fructose 6-phosphate | key enzyme of the glycolytic pathway | Methanocaldococcus jannaschii | AMP + D-fructose 1,6-bisphosphate | - |
? | |
ADP + D-fructose 6-phosphate | activity with GDP is 1.4% compared to the activity with ADP, activity with ATP is 0.3% compared to the activity with ADP, activity with GTP is 8.1% compared to the activity with ADP, no activity with diphosphate, phosphoenolpyruvate or polyphosphate | Methanocaldococcus jannaschii | AMP + D-fructose 1,6-bisphosphate | - |
? | |
ADP + D-fructose 6-phosphate | key enzyme of the glycolytic pathway | Methanocaldococcus jannaschii DSM 2661 | AMP + D-fructose 1,6-bisphosphate | - |
? | |
ADP + D-fructose 6-phosphate | activity with GDP is 1.4% compared to the activity with ADP, activity with ATP is 0.3% compared to the activity with ADP, activity with GTP is 8.1% compared to the activity with ADP, no activity with diphosphate, phosphoenolpyruvate or polyphosphate | Methanocaldococcus jannaschii DSM 2661 | AMP + D-fructose 1,6-bisphosphate | - |
? | |
D-fructose 6-phosphate + ADP | ADP can be replaced by acetylphosphate | Methanocaldococcus jannaschii | D-fructose 1,6-bisphosphate + AMP | - |
? | |
D-fructose 6-phosphate + ADP | ADP can be replaced by acetylphosphate | Methanocaldococcus jannaschii | D-fructose 1,6-bisphosphate + AMP | - |
ir |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 53362, calculated from sequence | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
ADP-dependent PFK | - |
Methanocaldococcus jannaschii |
ADP-Pfk | - |
Methanocaldococcus jannaschii |
MJ1604 | MJ1604 | Methanocaldococcus jannaschii |
pfkC | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
physiological function | key enzyme of the modified Embden-Meyerhof pathway of heterotrophic and chemolithoautotrophic archaea | Methanocaldococcus jannaschii |