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Literature summary for 2.7.1.145 extracted from
- Nucleoside analog cytotoxicity and bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in the nucleus or cytosol (2001), Biochem. Biophys. Res. Commun., 289, 229-233.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme can be used as a suicide gene in combined gene/chemotherapy treatment of cancer | Drosophila melanogaster |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of wild-type and mutant enzyme fused to GFP in cancer cell lines in the nucleus and cytosol, respectively | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | overexpression in cancer cell lines leads to increased cell sensitivity to several cytotoxic nucleoside analogues, expression of wild-type and mutant enzyme in an osteosarcoma cell line utilizing a replication-deficient retroviral vector reveals that the localization of the enzyme in cytosol or nucleus is not important for cell sensitivity and bystander cell killing | Drosophila melanogaster |
| R247S | site-directed mutagenesis, mutation abolishes nuclear import of the enzyme, the mutant enzyme is localized in the cytosol | Drosophila melanogaster |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | increased cell sensitivity to nucleoside analogues E-5-(2-bromovinyl)-2'-deoxyuridine and 1-beta-D-arabinofuranosylthymine in recombinant cancer cells overexpressing the enzyme in the nucleus or cytosol | Drosophila melanogaster |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Drosophila melanogaster | 5634 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, about, recombinant wild-type and mutant GFP-fusion protein, SDS-PAGE | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, about, recombinant wild-type and mutant GFP-fusion protein, SDS-PAGE | Drosophila melanogaster |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dm-dNK | - |
Drosophila melanogaster |
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