Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.144 extracted from

  • Bissett, D.L.; Anderson, R.L.
    Lactose and D-galactose metabolism in Staphylococcus aureus. III: Purification and properties of D-tagatose-6-phosphate kinase (1980), J. Biol. Chem., 255, 8745-8749.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NH4+ 33.3 mM activates Staphylococcus aureus

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during purification Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
Li+ 33.3 mM, 65% inhibition Staphylococcus aureus
Na+ 33.3 mM, 31% inhibition Staphylococcus aureus
NEM
-
Staphylococcus aureus
phosphate at high concentrations Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
D-tagatose 6-phosphate
-
Staphylococcus aureus
0.15
-
D-fructose 6-phosphate
-
Staphylococcus aureus
0.16
-
ATP
-
Staphylococcus aureus
0.17
-
ITP
-
Staphylococcus aureus
0.4
-
GTP
-
Staphylococcus aureus
0.9
-
UTP
-
Staphylococcus aureus
18
-
CTP
-
Staphylococcus aureus
20
-
TTP
-
Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ 8% of the activation with Mg2+, at 1.3 mM Staphylococcus aureus
Co2+ 54% of the activation with Mg2+, at 1.3 mM Staphylococcus aureus
K+ 33.3 mM activates Staphylococcus aureus
Mg2+ absolute requirement for a divalent cation, a Mg2+ : ATP ratio of 2.0-2.5 is required for maximal activity Staphylococcus aureus
Mn2+ 27% of the activation with Mg2+, at 1.3 mM Staphylococcus aureus
Ni2+ 15% of the activation with Mg2+, at 1.3 mM Staphylococcus aureus
Rb+ 33.3 mM activates Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
alpha2, 2 * 52000, SDS-PAGE Staphylococcus aureus
82000
-
gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-tagatose 6-phosphate Staphylococcus aureus inducible by D-galactose ADP + D-tagatose 1,6-bisphosphate
-
?
ATP + D-tagatose 6-phosphate Staphylococcus aureus involved in the catabolism of lactose and D-galactose ADP + D-tagatose 1,6-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.5
-
-
Staphylococcus aureus

Storage Stability

Storage Stability Organism
-20°C, 20 mM potassium phosphate buffer, pH 7.5, 20% v/v glycerol, 0.2% v/v 2-mercaptoethanol, stable for several months Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Staphylococcus aureus ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-tagatose 6-phosphate
-
Staphylococcus aureus ADP + D-tagatose 1,6-bisphosphate
-
?
ATP + D-tagatose 6-phosphate inducible by D-galactose Staphylococcus aureus ADP + D-tagatose 1,6-bisphosphate
-
?
ATP + D-tagatose 6-phosphate involved in the catabolism of lactose and D-galactose Staphylococcus aureus ADP + D-tagatose 1,6-bisphosphate
-
?
CTP + D-tagatose 6-phosphate
-
Staphylococcus aureus CDP + D-tagatose 1,6-bisphosphate
-
?
GTP + D-tagatose 6-phosphate
-
Staphylococcus aureus GDP + D-tagatose 1,6-bisphosphate
-
?
ITP + D-tagatose 6-phosphate
-
Staphylococcus aureus IDP + D-tagatose 1,6-bisphosphate
-
?
TTP + D-tagatose 6-phosphate
-
Staphylococcus aureus TDP + D-tagatose 1,6-bisphosphate
-
?
UTP + D-tagatose 6-phosphate
-
Staphylococcus aureus UDP + D-tagatose 1,6-bisphosphate
-
?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 52000, SDS-PAGE Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
in potassium phosphate buffer Staphylococcus aureus
8
-
in Hepes buffer Staphylococcus aureus
8.5
-
in glycylglycine buffer, bicine buffer and Tris buffer Staphylococcus aureus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5 pH 7.5: about 75% of maximal activity, pH 9.5: about 55% of maximal activity, glycylglycine buffer Staphylococcus aureus