Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | 33.3 mM activates | Staphylococcus aureus |
General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during purification | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | 33.3 mM, 65% inhibition | Staphylococcus aureus | |
Na+ | 33.3 mM, 31% inhibition | Staphylococcus aureus | |
NEM | - |
Staphylococcus aureus | |
phosphate | at high concentrations | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
D-tagatose 6-phosphate | - |
Staphylococcus aureus | |
0.15 | - |
D-fructose 6-phosphate | - |
Staphylococcus aureus | |
0.16 | - |
ATP | - |
Staphylococcus aureus | |
0.17 | - |
ITP | - |
Staphylococcus aureus | |
0.4 | - |
GTP | - |
Staphylococcus aureus | |
0.9 | - |
UTP | - |
Staphylococcus aureus | |
18 | - |
CTP | - |
Staphylococcus aureus | |
20 | - |
TTP | - |
Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 8% of the activation with Mg2+, at 1.3 mM | Staphylococcus aureus | |
Co2+ | 54% of the activation with Mg2+, at 1.3 mM | Staphylococcus aureus | |
K+ | 33.3 mM activates | Staphylococcus aureus | |
Mg2+ | absolute requirement for a divalent cation, a Mg2+ : ATP ratio of 2.0-2.5 is required for maximal activity | Staphylococcus aureus | |
Mn2+ | 27% of the activation with Mg2+, at 1.3 mM | Staphylococcus aureus | |
Ni2+ | 15% of the activation with Mg2+, at 1.3 mM | Staphylococcus aureus | |
Rb+ | 33.3 mM activates | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
alpha2, 2 * 52000, SDS-PAGE | Staphylococcus aureus |
82000 | - |
gel filtration | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-tagatose 6-phosphate | Staphylococcus aureus | inducible by D-galactose | ADP + D-tagatose 1,6-bisphosphate | - |
? | |
ATP + D-tagatose 6-phosphate | Staphylococcus aureus | involved in the catabolism of lactose and D-galactose | ADP + D-tagatose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.5 | - |
- |
Staphylococcus aureus |
Storage Stability | Organism |
---|---|
-20°C, 20 mM potassium phosphate buffer, pH 7.5, 20% v/v glycerol, 0.2% v/v 2-mercaptoethanol, stable for several months | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Staphylococcus aureus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-tagatose 6-phosphate | - |
Staphylococcus aureus | ADP + D-tagatose 1,6-bisphosphate | - |
? | |
ATP + D-tagatose 6-phosphate | inducible by D-galactose | Staphylococcus aureus | ADP + D-tagatose 1,6-bisphosphate | - |
? | |
ATP + D-tagatose 6-phosphate | involved in the catabolism of lactose and D-galactose | Staphylococcus aureus | ADP + D-tagatose 1,6-bisphosphate | - |
? | |
CTP + D-tagatose 6-phosphate | - |
Staphylococcus aureus | CDP + D-tagatose 1,6-bisphosphate | - |
? | |
GTP + D-tagatose 6-phosphate | - |
Staphylococcus aureus | GDP + D-tagatose 1,6-bisphosphate | - |
? | |
ITP + D-tagatose 6-phosphate | - |
Staphylococcus aureus | IDP + D-tagatose 1,6-bisphosphate | - |
? | |
TTP + D-tagatose 6-phosphate | - |
Staphylococcus aureus | TDP + D-tagatose 1,6-bisphosphate | - |
? | |
UTP + D-tagatose 6-phosphate | - |
Staphylococcus aureus | UDP + D-tagatose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 52000, SDS-PAGE | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
in potassium phosphate buffer | Staphylococcus aureus |
8 | - |
in Hepes buffer | Staphylococcus aureus |
8.5 | - |
in glycylglycine buffer, bicine buffer and Tris buffer | Staphylococcus aureus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | pH 7.5: about 75% of maximal activity, pH 9.5: about 55% of maximal activity, glycylglycine buffer | Staphylococcus aureus |