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Literature summary for 2.7.1.138 extracted from

  • Wijesinghe, D.S.; Brentnall, M.; Mietla, J.A.; Hoeferlin, L.A.; Diegelmann, R.F.; Boise, L.H.; Chalfant, C.E.
    Ceramide kinase is required for a normal eicosanoid response and the subsequent orderly migration of fibroblasts (2014), J. Lipid Res., 55, 1298-1309.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information generation of CERK-/- knockout mice Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens
Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + ceramide Homo sapiens
-
ADP + ceramide 1-phosphate
-
?
ATP + ceramide Mus musculus
-
ADP + ceramide 1-phosphate
-
?
ATP + ceramide Mus musculus BALB/c
-
ADP + ceramide 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8TCT0 gene CERK
-
Mus musculus Q8K4Q7 gene CERK
-
Mus musculus BALB/c Q8K4Q7 gene CERK
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast
-
Homo sapiens
-
fibroblast
-
Mus musculus
-
MEF cell primary mouse embryonic fibroblasts Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ceramide
-
Homo sapiens ADP + ceramide 1-phosphate
-
?
ATP + ceramide
-
Mus musculus ADP + ceramide 1-phosphate
-
?
ATP + ceramide
-
Mus musculus BALB/c ADP + ceramide 1-phosphate
-
?

Synonyms

Synonyms Comment Organism
CERK
-
Homo sapiens
CERK
-
Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens
ATP
-
Mus musculus

General Information

General Information Comment Organism
malfunction genetic ablation of CERK results in decreased levels of ceramide 1-phosphate in fibroblasts and inhibits the ability of fibroblasts to release arachidonic acid in response to mechanical trauma. Simulation of mechanical trauma of a wound by scratching a monolayer of fibroblasts from CERK+/+ mice demonstrates steadily increasing levels of arachidonic acid in a time-dependent manner in stark contrast to CERK-/- fibroblasts, reflected in scratch-induced eicosanoid levels. Loss of proper eicosanoid response translated into an abnormal migration pattern for the fibroblasts isolated from CERK-/-. Neither the wild-type nor the CERK-null fibroblasts demonstrated additional changes to the endogenous ceramide 1-phosphate content upon induction of mechanical trauma. The migratory response to wounding of CERK-/- fibroblasts can be rescued via the addition of exogenous eicosanoids Mus musculus
physiological function CERK is required for eicosanoid biosynthesis in response to mechanical insult. Proper migration of fibroblasts is one of the necessary steps of wound healing, requirement for the CERK-derived ceramide 1-phosphate in the proper healing response of wounds Mus musculus
physiological function CERK is required for eicosanoid biosynthesis in response to mechanical insult. Simulation of mechanical trauma of a wound demonstrates steadily increasing levels of arachidonic acid in a time-dependent manner reflected in scratch-induced eicosanoid levels. Proper migration of fi broblasts is one of the necessary steps of wound healing, requirement for the CERK-derived ceramide 1-phosphate in the proper healing response of wounds Homo sapiens