Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of CERK-/- knockout mice | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
Mg2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ceramide | Homo sapiens | - |
ADP + ceramide 1-phosphate | - |
? | |
ATP + ceramide | Mus musculus | - |
ADP + ceramide 1-phosphate | - |
? | |
ATP + ceramide | Mus musculus BALB/c | - |
ADP + ceramide 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8TCT0 | gene CERK | - |
Mus musculus | Q8K4Q7 | gene CERK | - |
Mus musculus BALB/c | Q8K4Q7 | gene CERK | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | - |
Homo sapiens | - |
fibroblast | - |
Mus musculus | - |
MEF cell | primary mouse embryonic fibroblasts | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ceramide | - |
Homo sapiens | ADP + ceramide 1-phosphate | - |
? | |
ATP + ceramide | - |
Mus musculus | ADP + ceramide 1-phosphate | - |
? | |
ATP + ceramide | - |
Mus musculus BALB/c | ADP + ceramide 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CERK | - |
Homo sapiens |
CERK | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | genetic ablation of CERK results in decreased levels of ceramide 1-phosphate in fibroblasts and inhibits the ability of fibroblasts to release arachidonic acid in response to mechanical trauma. Simulation of mechanical trauma of a wound by scratching a monolayer of fibroblasts from CERK+/+ mice demonstrates steadily increasing levels of arachidonic acid in a time-dependent manner in stark contrast to CERK-/- fibroblasts, reflected in scratch-induced eicosanoid levels. Loss of proper eicosanoid response translated into an abnormal migration pattern for the fibroblasts isolated from CERK-/-. Neither the wild-type nor the CERK-null fibroblasts demonstrated additional changes to the endogenous ceramide 1-phosphate content upon induction of mechanical trauma. The migratory response to wounding of CERK-/- fibroblasts can be rescued via the addition of exogenous eicosanoids | Mus musculus |
physiological function | CERK is required for eicosanoid biosynthesis in response to mechanical insult. Proper migration of fibroblasts is one of the necessary steps of wound healing, requirement for the CERK-derived ceramide 1-phosphate in the proper healing response of wounds | Mus musculus |
physiological function | CERK is required for eicosanoid biosynthesis in response to mechanical insult. Simulation of mechanical trauma of a wound demonstrates steadily increasing levels of arachidonic acid in a time-dependent manner reflected in scratch-induced eicosanoid levels. Proper migration of fi broblasts is one of the necessary steps of wound healing, requirement for the CERK-derived ceramide 1-phosphate in the proper healing response of wounds | Homo sapiens |