Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a kinase-dead mutant and a DELTAN-terminal deletion mutant of isozyme ITPKA, generation of Itpka N-terminal F-actin binding domain (N66-GFP) and the N66(L34P)-GFP mutants. N-terminal deletion mutants are fully active | Rattus norvegicus |
additional information | construction of a kinase-dead mutant and a DELTAN-terminal deletion mutant of isozyme ITPKB. N-terminal deletion mutants are fully active | Rattus norvegicus |
additional information | construction of a kinase-dead mutant and a DELTAN-terminal deletion mutant of isozyme ITPKC | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | - |
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P17105 | gene Itpka | - |
Rattus norvegicus | P42335 | gene Itpkb | - |
Rattus norvegicus | Q80ZG2 | gene Itpkc | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
PC-12 cell | - |
Rattus norvegicus | - |
pheochromocytoma cell | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ins(1,4,5)P3 kinase | - |
Rattus norvegicus |
ITPKA | - |
Rattus norvegicus |
Itpkb | - |
Rattus norvegicus |
Itpkc | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
malfunction | neurite length is significantly decreased in cells overexpressing isozymes Itpka and Itpkb but not Itpkc or IPMK. This result does not depend on the overexpression level of any of the kinases. PC-12 cells overexpressing GFP-tagged kinase-dead mutants Itpka/b have shorter neurites than GFP control cells | Rattus norvegicus |
malfunction | neurite length is significantly decreased in cells overexpressing isozymes Itpka and Itpkb but not Itpkc or IPMK. This result does not depend on the overexpression level of any of the kinases. PC12 cells overexpressing GFP-tagged kinase-dead mutants Itpka/b have shorter neurites than GFP control cells | Rattus norvegicus |
additional information | an F-actin binding site is located in the N-terminal part of isozyme Itpka. The catalytic activity is located at the C-terminal end, N-terminal deletion mutants are fully active | Rattus norvegicus |
additional information | an F-actin binding site is located in the N-terminal part of isozyme Itpkb. The catalytic activity is located at the C-terminal end, N-terminal deletion mutants are fully active | Rattus norvegicus |
additional information | neurite length is significantly decreased in cells overexpressing isozymes Itpka and Itpkb but not Itpkc or IPMK. This result does not depend on the overexpression level of any of the kinases | Rattus norvegicus |
physiological function | isozyme Itpka inhibits neurite outgrowth through both F-actin binding | Rattus norvegicus |
physiological function | isozyme Itpkb inhibits neurite outgrowth through both F-actin binding | Rattus norvegicus |
physiological function | isozyme Itpkc does not influence neurite length and neuron growth or F-actin binding | Rattus norvegicus |