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Literature summary for 2.7.1.127 extracted from

  • Koester, J.D.; Leggewie, B.; Blechner, C.; Brandt, N.; Fester, L.; Rune, G.; Schweizer, M.; Kindler, S.; Windhorst, S.
    Inositol-1,4,5-trisphosphate-3-kinase-A controls morphology of hippocampal dendritic spines (2016), Cell. Signal., 28, 83-90.
    View publication on PubMed
Search for more literature for 2.7.1.127

Cloned(Commentary)

Cloned (Comment) Organism
gene ITPKA, recombinnant expression of N-terminally GST-tagged isozyme ITPKA in Escherichia coli strain BL21 C41lys Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
synapse spine Mus musculus 45202
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Mus musculus
Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate Mus musculus
-
 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate Mus musculus C57BL/6
-
 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q8R071 gene Itpka
-
Mus musculus C57BL/6 Q8R071 gene Itpka
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally GST-tagged isozyme ITPKA from Escherichia coli strain BL21 C41lys by glutathione affinity chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
cervical spine
-
 Mus musculus
-
hippocampus
-
 Mus musculus
-
neuron isozyme inositol-1,4,5-trisphosphate-3-kinase-A is a neuron-specific, actin bundling protein concentrated at dendritic spines Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Mus musculus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Mus musculus C57BL/6 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
inositol-1,4,5-trisphosphate-3-kinase-A
-
 Mus musculus
ITPKA
-
 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus

General Information

General Information Comment Organism
malfunction ITPKA depletion in mice increases the number of hippocampal spine-synapses while reducing average spine length, depletion of ITPKA reduces the length of dendritic spines of hippocampal pyramidal cells Mus musculus
physiological function in addition to cross-linking actin filaments, ITPKA strongly inhibits Arp2/3-complex induced actin filament branching by displacing the complex from F-actin. n vivo ITPKA negatively regulates formation and/or maintenance of synaptic contacts in the mammalian brain. On the molecular level this effect appears to result from the ITPKA-mediated inhibition of Arp2/3-complex F-actin branching activity. ITPKA does not affect the F-actin bundling activity of drebrin A Mus musculus