Literature summary for 2.7.1.127 extracted from

Brehm, M.A.; Schreiber, I.; Bertsch, U.; Wegner, A.; Mayr, G.W.
Identification of the actin-binding domain of Ins(1,4,5)P3 3-kinase isoform B (IP3K-B) (2004), Biochem. J., 382, 353-362.

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Cloned(Commentary)

Cloned (Comment)	Organism
transient expression of isozyme B fragment 108-170 fused to EGFP in NRK 52E and PC12 cells co-localizes with F-actin, overexpression of isozyme B fused to EGFP in Escherichia coli, the enzyme co-localizes in with the cytoskeleton as well as with the endoplasmic reticulum and the plasma membrane, overexpression of inactive isozyme B lacking the F-actin target sequence at the noncatalytical N-terminus	Homo sapiens
transient expression of isozyme B in NRK 52E and PC12 cells, DNA and amino acid sequence determination and analysis, expression of isoform B comprising residues 555-934 as Strep-Tactin-tagged enzyme in Escherichia coli strain XL1-Blue, expression of isoform B comprising residues 108-170 as GST-fusion protein in Escherichia coli strain BL21(DE3)	Rattus norvegicus

Cloned (Comment)

Organism

transient expression of isozyme B fragment 108-170 fused to EGFP in NRK 52E and PC12 cells co-localizes with F-actin, overexpression of isozyme B fused to EGFP in Escherichia coli, the enzyme co-localizes in with the cytoskeleton as well as with the endoplasmic reticulum and the plasma membrane, overexpression of inactive isozyme B lacking the F-actin target sequence at the noncatalytical N-terminus

Homo sapiens

transient expression of isozyme B in NRK 52E and PC12 cells, DNA and amino acid sequence determination and analysis, expression of isoform B comprising residues 555-934 as Strep-Tactin-tagged enzyme in Escherichia coli strain XL1-Blue, expression of isoform B comprising residues 108-170 as GST-fusion protein in Escherichia coli strain BL21(DE3)

Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a mutant isozyme B by helix-breaking mutations in the actin binding domain and deletion of the actin binding domain in full-length isozyme B resulting in loss of actin binding ability	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoskeleton	the enzyme binds to F-actin in the cellular actin cytoskeleton with its actin binding domain at the N-terminus	Homo sapiens	5856	-
cytoskeleton	the enzyme binds to F-actin in the cellular actin cytoskeleton with its actin binding domain, within residues 108-170, at the N-terminus	Rattus norvegicus	5856	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isoform B	-
Rattus norvegicus	P42335	isoform B; isoform B	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	the enzyme is cleaved at the Asp-Pro clusters, residues 610, 617, and 765, at low pH and different temperatures producing 7 enzyme fragments of 17.4-82.3 kDa of the full-length enzyme of about 100 kDa, overview	Rattus norvegicus

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-fusion isoform B comprising residues 108-170 by glutathione affinity chromatography, recombinant truncated Strep-tagged isozyme B by affinity chromatography	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + 1D-myo-inositol 1,4,5-trisphosphate	-	Homo sapiens	ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate	-	?
ATP + 1D-myo-inositol 1,4,5-trisphosphate	-	Rattus norvegicus	ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate	-	?
additional information	the enzyme binds to F-actin in the cellular actin cytoskeleton with its F-actin domain at the N-terminus, the C-terminal fragment cannot bind to F-actin	Homo sapiens	?	-	?
additional information	the enzyme binds to F-actin in the cellular actin cytoskeleton with its F-actin domain at the N-terminus, the C-terminal fragment cannot bind to F-actin	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
Ins(1,4,5)P3 3-kinase isoform B	-	Homo sapiens
Ins(1,4,5)P3 3-kinase isoform B	-	Rattus norvegicus
IP3K-B	-	Homo sapiens
IP3K-B	-	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens
ATP	-	Rattus norvegicus