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Literature summary for 2.7.1.127 extracted from
- Calcium-calmodulin-dependent protein kinase II and protein kinase C-mediated phosphorylation and activation of D-myo-inositol 1,4, 5-trisphosphate 3-kinase B in astrocytes (1999), J. Biol. Chem., 274, 14734-14742.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 12-O-tetradecanoylphorbol-13-acetate | 2fold activation, isoform B | Homo sapiens |  |
| 12-O-tetradecanoylphorbol-13-acetate | 2fold activation, isoform B | Rattus norvegicus | |
| CaM kinase II | phosphorylation of isoenzyme B by calmodulin kinase II and protein kinase C added together results in a maximal 60-70fold activation, no effect on the sensitivity to the Ca2+/calmodulin complex, CaM kinase II alone activates 35-40fold in the presence of Ca2+ and calmodulin | Homo sapiens | |
| CaM kinase II | phosphorylation of isoenzyme B by calmodulin kinase II and protein kinase C added together results in a maximal 60-70fold activation, no effect on the sensitivity to the Ca2+/calmodulin complex, CaM kinase II alone activates 35-40fold in the presence of Ca2+ and calmodulin | Rattus norvegicus | |
| Carbachol | calphostin C, KN-93 or KN-62 partially prevents activation | Rattus norvegicus | |
| Carbachol | 6-8fold okadaic acid-sensitive activation, maximal at 0.01 mM, isoform B, carbachol-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction | Homo sapiens | |
| Carbachol | 6-8fold okadaic acid-sensitive activation, maximal at 0.01 mM, isoform B, carbachol-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction | Rattus norvegicus | |
| additional information | isoenzyme B of 1321N1 cells is not activated by UTP | Homo sapiens | |
| additional information | isoenzymes A and B are not activated by protein kinase A | Rattus norvegicus | |
| Protein kinase C | phosphorylation of isoenzyme B by calmodulin kinase II and protein kinase C added together results in a maximal 60-70fold activation, but protein kinase C alone inhibits in the presence of Ca2+ and calmodulin, no effect on the sensitivity to the Ca2+/calmodulin complex | Homo sapiens | |
| Protein kinase C | phosphorylation of isoenzyme B by calmodulin kinase II and protein kinase C added together results in a maximal 60-70fold activation, but protein kinase C alone inhibits in the presence of Ca2+ and calmodulin, no effect on the sensitivity to the Ca2+/calmodulin complex | Rattus norvegicus | |
| UTP | - |
Homo sapiens | |
| UTP | 6-8fold okadaic acid-sensitive activation, maximal at 0.01 mM, isoform B, calphostin C, KN-93 or KN-62 partially prevents activation, UTP-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Protein kinase C | - |
Homo sapiens | |
| Protein kinase C | protein kinase C alone, without CaM kinase II, inhibits in the presence of Ca2+ and calmodulin | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
1D-myo-inositol 1,4,5-trisphosphate | isoform B | Homo sapiens | |
| 0.0015 | - |
1D-myo-inositol 1,4,5-trisphosphate | isoform B | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| particle-bound | not activated isoenzyme B: 65% soluble, 35% particulate fraction, carbachol-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction, only 10% remain soluble | Homo sapiens | - |
- |
| particle-bound | not activated isoenzyme B: 65% soluble, 35% particulate fraction, carbachol-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction, only 10% remain soluble | Rattus norvegicus | - |
- |
| soluble | unstimulated isoenzyme B: 65% soluble, 35% particulate fraction, carbachol-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction, only 10% remain soluble | Homo sapiens | - |
- |
| soluble | unstimulated isoenzyme B: 65% soluble, 35% particulate fraction, carbachol-activated isoenzyme B shows a redistribution of enzyme from soluble to particulate fraction, only 10% remain soluble | Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | phosphorylation of isoenzyme B has no effect on the sensitivity to the Ca2+/calmodulin complex | Rattus norvegicus | |
| Ca2+ | activation by Ca2+/calmodulin | Homo sapiens | |
| Ca2+ | activation by Ca2+/calmodulin | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
x * 88000, isoform B, SDS-PAGE, Western blot analysis | Homo sapiens |
| 88000 | - |
x * 88000, isoform B, SDS-PAGE, Western blot analysis | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | Homo sapiens | regulatory mechanism of isoenzyme B involving phosphorylation by both protein kinase C and CaM kinase II | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | regulatory mechanism of isoenzyme B involving phosphorylation by both protein kinase C and CaM kinase II | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | Homo sapiens | inositol 1,4,5-trisphosphate is a second messenger | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | inositol 1,4,5-trisphosphate is a second messenger | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isoform B | - |
| Rattus norvegicus | - |
2-3 days old, isoform B | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phosphorylation of enzyme by calmodulin kinase II and protein kinase C added together results in a maximal 60-70fold activation, distinct sites are phosphorylated independently by both protein kinases, prevented by the CaM kinase II inhibitors KN-93, KN-62 and the protein kinase C inhibitor calphostin C | Homo sapiens |
| phosphoprotein | phosphorylation of enzyme by calmodulin kinase II and protein kinase C added together results in a maximal 60-70fold activation, distinct sites are phosphorylated independently by both protein kinases, prevented by the CaM kinase II inhibitors KN-93, KN-62 and the protein kinase C inhibitor calphostin C | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzyme B | Homo sapiens |
| isoenzyme B | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| astrocyte | cerebral cortex astrocytes, isoform B | Rattus norvegicus | - |
| astrocytoma cell | 1321N1 cells, isoform B | Homo sapiens | - |
| brain | - |
Homo sapiens | - |
| brain | cortical astrocytes, isoform B | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.4 | - |
isoform B, in absence of the Ca2+/calmodulin complex | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | regulatory mechanism of isoenzyme B involving phosphorylation by both protein kinase C and CaM kinase II | Homo sapiens | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | regulatory mechanism of isoenzyme B involving phosphorylation by both protein kinase C and CaM kinase II | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | inositol 1,4,5-trisphosphate is a second messenger | Homo sapiens | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | inositol 1,4,5-trisphosphate is a second messenger | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 88000, isoform B, SDS-PAGE, Western blot analysis | Homo sapiens |
| ? | x * 88000, isoform B, SDS-PAGE, Western blot analysis | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
| ATP | - |
Rattus norvegicus | |
| Calmodulin | activation by Ca2+/calmodulin | Homo sapiens | |
| Calmodulin | activation by Ca2+/calmodulin | Rattus norvegicus | |
| Calmodulin | phosphorylation of isoenzyme B has no effect on the sensitivity to the Ca2+/calmodulin complex | Rattus norvegicus | |
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